Sonication-Assisted Agrobacterium-mediated Gene Transformation of Oil Palm Secondary Somatic Embryo

บทคัดย่อ

The infl uence was investigated of the hygromycin concentration on the survival rate of oil palm secondary somatic embryos (SSEs) and sonication-assisted Agrobacterium-mediated transformation (SAAT). The results showed that 20 mg.L-1 hygromycin could completely inhibit growth of SSEs, suitable for selection of transformed SSEs. The transient ß-glucuronidase (gus) expression was affected by the different period of SAAT which was signifi cantly enhanced by increasing the period of SAAT. However, the longer exposure to SAAT caused a decrement in the frequency of plantlet regeneration. The highest transformation effi ciency was obtained when the SSEs were sonicated for 5 min. Polymerase chain reaction and Southern blot hybridization analysis confi rmed the presence of the reporter genes in the transformed plantlet genome.