Development of Immunological Test Kit for the Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Swine

Authors

  • Surasak Jittavisutthikul Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom 7314, Thailand.
  • Amara Thongpan Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Orawan Boodde Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Wilairat Shumsing Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Worawidh Wajjwalku Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

PRRS, fusion nucleocapsid protein, checkerboard titration, cut-off value, partial purified, k value, ELISA

Abstract

               Two types of porcine reproductive and respiratory syndrome virus (PRRSV), European (EU) and North American (US), cause an important swine disease called porcine reproductive and respiratory syndrome (PRRS). An enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of serum antibodies against these two PRRS was developed. A fused protein containing EU PRRSV and US PRRSV nucleocapsid proteins (USEU-rN protein) expressed in Escherichia coli were partial purified and used as antigens.

               As determined by a checkerboard titration, an optimal dilution of the USEU-rN protein and serum were 1:1600 and 1:40, respectively. The optimal cut-off value for the developed USEU PRRS ELISA was 0.4, with diagnostic sensitivity and specificity of 97.5% and 100%, respectively. Using
Two Graph-ROC program testing with 200 positive sera (IDEXX® HerdCheck PRRS ELISA) and with 200 negative sera from PRRS-free country (Denmark), comparison of 1,077 field sera obtained from USEU PRRS ELISA with those from IDEXX® ELISA showed the degree of agreement (k value) was highly obtained at 0.7916. The kit is considered reliable for routine diagnosis, epidemiological surveys and outbreak investigations.

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Published

2008-03-30

How to Cite

Surasak Jittavisutthikul, Amara Thongpan, Orawan Boodde, Wilairat Shumsing, and Worawidh Wajjwalku. 2008. “Development of Immunological Test Kit for the Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Swine”. Agriculture and Natural Resources 42 (1). Bangkok, Thailand:19-30. https://li01.tci-thaijo.org/index.php/anres/article/view/244409.

Issue

Vol. 42 No. 1 (2008): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.