A Simple Method to Extract Mitochondrial DNA in a Non-invasive Phylogenetic Study of Domestic Native Thai Ducks

Authors

  • Piyanan Leekaew Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Thaweesak Songserm Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom 73140, Thailand.
  • Apassara Choothesa Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Ukadej Boonyaprakob Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

alkaline extraction, Anas platyrhynchos, Mallard, mtDNA, phylogenetics

Abstract

A simple low-cost method for extracting mitochondrial DNA (mtDNA) from a single plucked feather for a non-invasive genetic study of ducks is described. The mtDNA was isolated via alkaline lysis, and after neutralization, the crude DNA-containing lysate was used directly for PCR. We have had a 100% (30/30) success in amplification of a 710-bp fragment of the mtDNA control D-loop region. Sequence analysis and phylogenetic relationship of the 667-bp D-loop segments of the two domestic native Thai ducks, Nakorn-Pathom (NP) and Park-Nam (PN), was further investigated. Two breeds exhibited 100% identity over the entire 667-bp products, although several types of mtDNA polymorphism were detected. Comparing the 667-bp D-loop sequence data with those of Anas ducks and Muscovy duck used as an outgroup showed that both native Thai ducks and Mallard (Anas platyrhynchos) haplotype A clustered together (bootstrap support 91%), indicating that there was common ancestor for those
breeds. Here we report a quick, simple, reliable and inexpensive DNA extraction procedure for a duck’s feather. Our findings clearly demonstrate that the domestic native Thai ducks derive their origin from the Mallard group A haplotypes.

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Published

2008-03-30

How to Cite

Piyanan Leekaew, Thaweesak Songserm, Apassara Choothesa, and Ukadej Boonyaprakob. 2008. “A Simple Method to Extract Mitochondrial DNA in a Non-Invasive Phylogenetic Study of Domestic Native Thai Ducks”. Agriculture and Natural Resources 42 (1). Bangkok, Thailand:41-50. https://li01.tci-thaijo.org/index.php/anres/article/view/244412.

Issue

Vol. 42 No. 1 (2008): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.