Application of Inter Simple Sequence Repeat (ISSR) Marker for Typing of Saccharomycopsis fibuligera Isolated from Loog-pang, Kao-mag and Satho

Abstract

The D1/D2 26S rDNA sequence was shown to be applicable for yeast species identification. The size of this region was 600 bp and the identification results showed more than 98% similarity of all isolates compared to the sequences in the Genbank database. This research also included a small survey for ISSR variation in the yeast Saccharomycopsis fibuligera. Eight of twelve UBC (University of British Columbia) primers gave 80 reproducible patterns. The size of the ISSR products ranged from 300 to about 2000 bp. Among the eight primers used in this study, the primers in the UBC group (880/888/889) gave the most informative profiles for grouping and distinguished 11 S. fibuligera isolates into six groups. The two isolates in groupVI were further divided from others by UBC 891. Only one of the primers in the UBC group together with UBC 891 was enough to discriminate the 11 S. fibuligera isolates into seven groups. These results indicated that the ISSR marker could be successfully applied to study intra-specific variation and that there were genetic variations in the yeast species of S. fibuligera from different kinds of food products in Thailand.