PCR-based Restriction Fragment Length Polymorphism for Subtyping of Salmonella from Chicken Isolates

Authors

  • Han Yu Jong Academy of Health and Food Science, Pyongyang, DPR of Korea.
  • Pak Thae Su Academy of Health and Food Science, Pyongyang, DPR of Korea.
  • Pannatee Sanpong Interdisciplinary Graduate Program in Genetic Engineering, Kasetsart University, Bangkok 10900, Thailand.
  • Worawidh Wajjwalku Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Thavajchai Sukpuaram Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Patamaporn Amavisit Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Salmonella, PCR-based RFLP, flagella, subtyping

Abstract

Genotypic diversity in two flagellin genes, fliC and fljB, encoding phase-1 and phase-2 flagellin of Salmonella enterica, offers a potential biomarker for Salmonella subtyping. Forty-seven Salmonella isolates of 20 different serovars derived from chicken samples in Thailand were studied using the fliC/fljB PCR-based RFLP assay. With two restriction endonucleases, MboI and HhaI, the fliC showed 11 and 9 patterns, while the fljB showed 6 and 7 patterns respectively. Though the PCR-based RFLP test cannot replace serotyping, the assay is based on the flagellin genes encoding proteins on the bacterial surface that are related to serotyping scheme. Overall, the assay was reproducible and successfully applied to simply screen Salmonella serovars as an alternative subtyping test for rapid traceability of Salmonella contamination in chicken production.

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Published

2010-02-28

How to Cite

Han Yu Jong, Pak Thae Su, Pannatee Sanpong, Worawidh Wajjwalku, Thavajchai Sukpuaram, and Patamaporn Amavisit. 2010. “PCR-Based Restriction Fragment Length Polymorphism for Subtyping of Salmonella from Chicken Isolates”. Agriculture and Natural Resources 44 (1). Bangkok, Thailand:79-83. https://li01.tci-thaijo.org/index.php/anres/article/view/244883.

Issue

Vol. 44 No. 1 (2010): January-February

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.