Growth Reduction of Papaya Plantlets for In Vitro Germplasm Preservation

บทคัดย่อ

Papaya plantlets, line Pb1-R1, were cultured on modified on Murashige and Skoog media supplemented with different concentration of chloro choline chloride (CCC) namely 0, 50, 100, 250,500, 1000, 1250, 1500, 1750 and 2000 mg/l and cultured under 4 levels of temperature namely 4°C, 10'C, 15'C and 27°C. Under room temperature, papaya plantlets cultured on media supplemented with each level of CCC could grow for 3 months. On the other hand, under low temperature, 4°C 10°C and 15°C, papaya plantlets could grow for 6 months. Culture papaya plantlets under 15°C without CCC is the suggested condition for preservation of papaya germplasm for 6 months.