Selection of Thermophilic Xylanase Producing Mold

บทคัดย่อ

A total of 238 mold cultures capable in utilizing xylan as sole source of carbon were isolated from decomposed lignocellulosic materials. Based on the ability to produce high xylanase activity at 45 ^oC, 65 isolates were selected. All cultures produced enzymes which hydrolyzed xylan to xylose and xylo-oligosaccharides. The most efficient strain, designated No. 4-45-1F produced as high as enzyme 36.6 units/ml. Regarding to the screening procedures, it was found that activity of the enzyme produced by the cultures was not quantitatively related to size of the clear zones which was developed by flooding the xylan agar plate with iodine solution. All the selected cultures belong to the genus Aspergillus. The best strain was identified as Aspergillus fumigutus Fresenius.