Antibiotic resistance in Escherichia coli isolated from manure in dairy and beef cattle

Abstract

The aim of this study was to compare the association of antibiotic resistance (AR) of Escherichia coli with the dairy-beef cattle groups. Manure samples were randomly collected from Dairy group (Dg)
4 samples, and Beef cattle group (Bg) 3 samples. Standard Plate Count method was used to determine the coliform concentrate, and Disk Diffusion method was used to assess the presence of the multidrug-resistant to E. coli (Oxytetracycline, Penicillin G, Sulfamethoxazole, Streptomycin, and Enrofloxacin). The results from Standard Plate Count method showed that the mean of coliform concentrate was Dg 2.75 log CFU/g, and Bg 3.96 log CFU/g. The results from testing multidrug-resistant of E. coli indicated that Dg was 30% of AR and 70% of non-AR resistance, while Bg was 56.67% of AR, and 43.33% of non-AR. In conclusion, the coliform concentrate of the Bg was greater than the Dg 4.07 log CFU/g (p=0.014) by T-test statistics. There was no association between antibiotic resistance and the dairy-beef cattle groups by Chi-square- test