The effect of dietary vitamin E supplementation on semen quality and fertility rate in native chicken
Keywords:
vitamin E, semen quality, fertility, native chickenAbstract
The objective of this study was to investigate the effect of vitamin E supplementation on semen quality and fertility rate in Thai native chickens. The experiment was divided into two parts as follows: Part 1: The effect of dietary vitamin E supplementation on semen quality. Twelve Thai native roosters aged 1–2 years were used. Semen was collected twice a week for 12 weeks. The roosters were divided into three treatment groups receiving dietary vitamin E at levels of 0, 50, and 100 mg/kg of feed. The results showed that forward motility, sperm viability, and semen concentration were significantly higher in all vitamin E-supplemented groups compared to the control group (p<0.05). The percentage of abnormal sperm was significantly lower in all vitamin E-supplemented groups (p<0.05). Moreover, the semen volume was highest in the group supplemented with 100 mg/kg of vitamin E. Part 2: The effect of dietary vitamin E supplementation on fertility rate. Thirty-six Thai native hens aged 8–12 months were used. They were divided into three treatment groups receiving vitamin E at levels of 0, 50, and 100 mg/kg of feed. Each group was inseminated with semen obtained from roosters that had been supplemented with the same level of vitamin E. The study found that the fertility rate and hatchability of fertile eggs were significantly higher in the group supplemented with 100 mg/kg of vitamin E (p<0.05), while the percentage of embryonic mortality was the lowest. In conclusion, the results of this study indicate that vitamin E supplementation enhances reproductive performance in both male and female Thai native chickens.
References
Abd El-Ghany, W. (2022). Vitamin E and its impact on poultry production: An update. Journal of the Hellenic Veterinary Medical Society, 73(1), 3571-3582. https://doi.org/10.12681/jhvms.25836
Abioja, M.O., Apuu, S., Daramola, J.O., Wheto, M., & Akinjute, O.F. (2023). Semen quality and sperm characteristics in broiler breeder cockerels fed vitamin E during hot season. Acta Scientiarum. Animal Sciences, 45, 1-9. https://doi.org/10.4025/actascianimsci.v45i1.56848
Amevor, F.K., Cui, Z., Du, X., Ning, Z., Shu, G., Jin, N., Deng, X., Tian, Y., Zhang, Z., Kang, X., Xu, D., You, G., Zhang, Y., Li, D., Wang, Y., Zhu, Q., & Zhao, X. (2021). Combination of quercetin and vitamin E supplementation promotes yolk precursor synthesis and follicle development in aging breeder hens via liver-blood-ovary signal axis. Animals, 11(1915), 1-24. https://doi.org/10.3390/ani11071915
Amevor, F.K., Cui, Z., Du, X., Ning, Z., Shu, G., Du, X., Jin, N., Deng, X., Xu, D., Tian, Y., Zhang, Y., Li, D., Wang, Y., Du, X., & Zhao, X. (2022). Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens. Poultry Science, 101, 101851. https://doi.org/10.1016/j.psj.2022.101851
Barnes, D.A., Thurston, T.R., Scott, T.R., & Korn, N. (1996). Effect of added spermiophages in pooled turkey semen on fertility, embryonic mortality, and hatchability. Poultry Science, 75(7-9), 943-948. https://doi.org/10.3382/ps.0750943
Birkett, J.R. (1981). Preservation of the fertilizing capacity of cock semen incubated In Vitro. At 41 C. Poultry Science, 60(5-6), 1075-1078. https://doi.org/10.3382/ps.0601075
Biswas, A., Mohan, J., & Sastry, K.V. (2009). Effect of higher dietary vitamin E concentrations on physical and biochemical characteristics of semen in Kadaknath cockerels. British Poultry Science, 50(6), 733-738. https://doi.org/10.1080/00071660903264369
Chantalakhana, C. (1997). Experimental principles. Statistics, analysis methods and research design. Department of Animal Science, Faculty of Agriculture, Kasetsart University. (In Thai)
Danikowski, S., Sallmann, H.P., Hallo, I., & Flachowsky, G. (2002). Influence of high levels of vitamin E on semen parameters of cocks. Journal of Animal Physiology and Animal Nutrition (Berl), 86(11-12), 376-82. https://doi.org/10.1046/j.1439-0396.2002.00396.x
Donoghue, A.M., & Donoghue, D.J. (1997). Effect of water-and lipid-soluble antioxidants on turkey sperm viability, membrane integrity, and motility during liquid storage. Poultry Science, 76(10), 1440-1455. https://doi.org/10.1093/ps/76.10.1440
Gore, A.B., & Qureshi, M.A. (1997). Enhancement of humorol and cellular immunity by vitamin E after embryonic exposure. Poultry Science, 76(7), 984-991. https://doi.org/10.1093/ps/76.7.984
Hemly, N.I.M., Zainuddin, N.N., Muhammad, A.I., Chwen, L.T., & Samsudin, A.A. (2023). Supplementation of different sources of selenium on laying performance, egg quality traits, and shell calcification expressions in 50 weeks Lohmann Brown layer hen reproductive tract. Veterinary Integrative Sciences, 21(3), 677-692. https://doi.org/10.12982/VIS.2023.049
Horwitt, M.K. (1986). The promotion of vitamin E. The Journal of Nutrition, 116(7), 1371-1377. https://doi.org/10.1093/jn/116.7.1371
Khan, R.U. (2011). Antioxidants and poultry semen quality. World’s Poultry Science Journal, 67(2), 297-308. https://doi.org/10.1017/S0043933911000316
Khan, R.U., Rahman, Z.U., Javed, I., & Muhammad, F. (2012). Effect of vitamins, probiotics and protein on semen traits in post-molt male broiler breeders. Animal Reproduction Science, 135(1-4), 85-90. https://doi.org/10.1016/j.anireprosci.2012.09.005
Laopaiboon, B., & Duangjinda, M. (2012). Thai native chicken: Past, present and future. Khon Kaen Agriculture Journal, 40, 309-312. (In Thai)
Lertsansiri, K., Haitook, T., Khammeng, T., & Phaengphairee, P. (2015). Effect of raising systems and locations on reproduction performance of native chickens in rural condition. Khon Kaen Agriculture Journal, 43(Suppl. 1), 20-25. (In Thai)
Lin, Y.F., Chang, S.J., & Hsu, A.L. (2004). Effects of supplemental vitamin E during the laying period on the reproductive performance of Taiwan native chickens. British Poultry Science, 45(6), 807-814. https://doi.org/10.1080/00071660400012717
Lin, Y.F., Chang, S.J., Yang, J.R., Lee, Y.P., & Hsu, A.L. (2005). Effects of supplemental vitamin E during the mature period on the reproduction performance of Taiwan native chicken cockerels. British Poultry Science, 46(3), 366-373. https://doi.org/10.1080/00071660500098186
Liu, Y.J., Zhao, L.H., Mosenthin, R., Zhang, J.Y., Ji, C., & Ma, Q.G. (2019). Protective effect of vitamin E on laying performance, antioxidant capacity, and immunity in laying hens challenged with Salmonella enteritidis. Poultry Science, 98(11), 5847-5854. https://doi.org/10.3382/ps/pez227
Lukaszewicz, E. (2001). Effects of semen filtration and dilution rate on morphology and fertility of frozen gander spermatozoa. Theriogenology, 55(9), 1819-1829. https://doi.org/10.1016/S0093-691X(01)00524-6
Mangiagalli, M.G., Bava, L., Toschi, I., Roveda, P., Vezzoli, G., & Castrovilli, C. (2004). Effect of addition to the diet of linseed oil and vitamin E on semen quality in cock. Rivista di Avicoltura, 73(1), 38-40.
Pimcomelai, O., Prapasawat, C., & Leotaragul, A. (2006). Production performance of native chickens in village. Journal of Agriculture, 22(2), 171-177. (In Thai)
Rengaraj, D., & Hong, Y.H. (2015). Effects of dietary vitamin E on fertility functions in poultry species. International Journal of Molecular Sciences, 16, 9910-9921. https://doi.org/10.3390/ijms16059910
Sexton, T.J. (1977). A new poultry semen extender, effect of extender on the fertility of chicken semen. Poultry Science, 56(5), 1443-1446. https://doi.org/10.3382/ps.0561443
Sriboonlue, P. (1997). Vitamins and coenzymes. In P. Sriboonlue, S. Wongkham, P. Boonsiri & P. Koonanuwatchaidet (Eds.), Biochemistry textbook. Department of Biochemistry, Faculty of Medicine, Khon Kaen University. (In Thai)
Surai, P.F., Kostjuk, I., Wishart, G., Macpherson, A., Speake, B., Noble, R., Ionov, I., & Kutz, E. (1998). Effect of vitamin E and selenium supplementation of cockerel diets on glutathione peroxidase activity and lipid peroxidation susceptibility in sperm, testes and liver. Biological Trace Element Research, 64(1-3), 119-132. https://doi.org/10.1007/BF02783329
Surai, P.F., Kutz, E., Wishart, G.J., Noble, R.C., & Speake, B.K. (1997). The relationship between the dietary provision of α-tocopherol and the concentration of this vitamin in the semen of chicken: effects on lipid composition and susceptibility to peroxidation. Journal Reproduction and Fertility, 110(1), 47-51.
Surai, P.F., Sparks, N.H.C., Speake, B.K., & Cerolini, S. (2005). Dietary fish and evening primrose oil with vitamin E effects on semen variables in cockerels. British Poultry Science, 46(2), 214-222. https://doi.org/10.1080/00071660500065839
Tangtaweewipat, S., Cheva-Isarakul, B., Panyachatraksa, K., Panyavi, S., Nachiangmai, P., Sangdaoruang, S., & Sirisrisawut, B. (2004). The production improvement of native chickens: commercial production system. Complete Research Report. Chiang Mai University. (In Thai)
Voorst, A.V., & Leenstra, F.R. (1995). Fertility rate of daily collected and cryopreserved fowl semen. Poultry Science, 74(1), 136-140. https://doi.org/10.3382/ps.0740136
Zanini, S.F., Torres, C.A., Bragagnolo, N., Turatti, J.M., Silva, M.G., & Zanini, M.S. (2003). Evaluation of the ratio of omega6 : omega3 fatty acids and vitamin E levels in the diet on the reproductive performance of cockerels. Archives of Animal Nutrition, 57(6), 429-442. https://doi.org/10.1080/0003942032000161072
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