Accumulation of 1-deoxynojirimycin (DNJ) in Different Species of Silkworm Larvae in Northeast of Thailand
Keywords:DNJ, Accumulation, Thailand
This work aims to investigate 1-deoxynojirimycin (DNJ, a potential antiglucosidase) accumulation in different species of silkworm larvae in northeast of Thailand. Twenty-nine species were cultivated at the Queen Sirikit Department of Sericulture (Sakon Nakhon, Thailand); silkworm specie; SP1, SP2, SP3, N.K.02, N.K.04, N.K.08, N.K.011, SR.5, R.A.05, Chok Amnuai, Non Rue Si, Haujaraud, Kaew Sakon, Sumloung, Vietnam, Vanasavan, Ai Tang, Kuang Wan, Nuangnoi Srisaket, Srisaket1, Tabtim Siam06, Kaki, NuangTui, Nuang Lai, Nuang Kaw, Nuang On Yaung, Naung Saew, Nuang Daeng and Nuang Luang. The silkworm larvae were freely fed with mature mulberry leaves (variety Buriram 60) as common feeding silkworms for sericulture industries. The silkworm’s age of 12, 17 and 26 days were investigated DNJ accumulation using HPLC. The results found that silkworms species SR.5 (113±7.5 mg/100g), N.K.04 (97±9.3 mg/100g) and Nuangnoi Srisaket (76±8.2 mg/100g) had high DNJ content at age of 12 days. Kuang wan (111±9.4 mg/100g), Kaki (104±9.3 mg/100g) and Nuangnoi Srisaket (103±6.6 mg/100g) had high DNJ accumulation at 17 days while Nuangnoi Srisaket (96±7.5 mg/100g), Srisaket1 (91±8.1 mg/100g), SP1 (91±9.6 mg/100g) had high DNJ content at 26 days, respectively. As results, DNJ content in silkworms have not relevant on age difference. Considering to DNJ amount, there are several silkworm species had high DNJ (around 100 mg/100g) that was close to DNJ amount in mulberry supplement produced from shoot or young mulberry leaves, and could be applied for supplementary foods
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