Effect of yeast extract combination with fish extract organic fertilizer on growth of Dendrobium aphyllum in vitro culture

Abstract

     This research aimed to know the effect of yeast extract combination with fish extract organic fertilizer on growth of Dendrobium aphyllum in-vitro culture for plantlets production of medicinal orchid under organic system and save cost. The various concentrations of yeast extract (0, 1, 1.5, 2, 2.5 or 3 g/l) were added in each treatment which contain 2 ml/l of fish extract organic fertilizer and they were compared with VW media (control). All treatments were supplemented with 150 ml/l coconut water, 100 g/l banana pulp, 20 g/l sugar and 1 g/l activated charcoal. The result showed that the stem number of D. Aphyllum was highest when their shoots were cultured in media which contain with 3 g/l yeast extract, while their stem growth and number of root were not significantly different in each treatment. The longest root length was obtained when they were cultured in VW media for 30-60 days and data was significantly different with other treatments. Therefore, this present study cloud be concluded that we can use the media which contained 2 ml/l fish extract organic fertilizer and add with 3 g/l yeast extract instant of VW media for D. aphyllum in-vitro culture and this media should be applied with 150 ml/l coconut water, 100 g/l banana pulp, 20 g/l sugar and 1 g/l activated charcoal to enhance the growth of plant.