Comparison on Using Organic Supplements on Growth of 2 in vitro Dendrobium sp. Seedlings

Abstract

     The research aimed to investigate the effect of modified culture medium on growth of in vitro seedling of Dendrobium orchids. The modified culture media were prepared by using organic matters including potato extract (150 g/l) tomato extract (75 g/l) fish extract fertilizer (2 ml/l) supplemented in without and with KNO₃. These modified culture media were compared with VW medium (Vacin & Went) for in vitro growth of Dendrobium moschatum (Buch.-Ham.) Sw. and Dendrobium primulinum Lindl. The growth of seedling was investigated for 3 months after culturing. The results showed that VW medium was better and suitable for long time of growth in these 2 species but the cost of medium was high (about 60 Bath per liter). Whereas, the growth of these orchids in modified culture media which including the potato extract [medium supplemented with potato extract (150 g/l) + KNO₃ (1.25 g/l), medium supplement with potato extract (150 g/l) + tomato extract (75 g/l) + fish extract fertilizer (2 ml/l) and medium supplement with potato extract (150 g/l) + fish extract fertilizer (2 ml/l) + KNO₃ (1.25 g/l)] were not different to VW medium. While, the cost of these modified culture media was low (about 25-28 Bath per liter) but they have limitation on culturing period when compared with VW medium. However, these modified culture media can be applied for in vitro culture of these orchids because of save cost and simple practice.