Effect of Spawn Preparation and Pretreatments of Substrate on Yield of Hungarian Oyster Mushroom
Article Sidebar
Main Article Content
Abstract
The research attempted to develop a simple technique for growing bagged mushrooms. The traditional methodology was compared to the modified methods for making Hungarian oyster mushrooms (Pleurotus ostreatus) mother spawn. The results showed that culture from the modified method cultured fresh mushroom tissue on sorghum disinfected with hydrogen peroxide (H2O2) mycelial took a longer period to complete invasion than the traditional method. However, after 14 days of cultivation, mycelial density was found to be higher. This method was chosen as the mother spawn in the next experiment. The experiment was conducted using rice straw as the main substrate and provided different substrate preparation method, i.e. steaming, boiling, soaking in different water, H2O2 solution, and gypsum solution. The results showed that boiling rice straw results in the time to flowering (26 days) and the mushrooms yield (385 g.) was statistically significantly lower than other methods. This was consistent with the results of the analysis of the nutrient value of rice straw. Soaking rice straw in plain water had the highest statistically significant percentage of contamination.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
กรมส่งเสริมการเกษตร. 2558. การเพาะเห็ดเบื้องต้น. ชุมนุมสหกรณ์การเกษตรแห่งประเทศไทย, กรุงเทพฯ. ธัญญา ทะพิงค์แก. 2553. การผลิตเชื้อเห็ดนางรมแบบง่าย.รายงานประชุมวิชาการเกษตร ครั้งที่ 11, มหาวิทยาลัยขอนแก่น 25-26 มกราคม. มหาวิทยาลัยขอนแก่น, ขอนแก่น. น. 361-364.
ธัญญา ทะพิงค์แก. 2561. การเพาะเห็ดถั่งเช่าเป็นอาชีพ. มิตรเกษตรการตลาดและโฆษณา, กรุงเทพฯ.
พรวิภา โสภณพัฒนะโภคา สมชาย สุขะกูล และประภาพร ตั้งกิจโชติ. 2556. ประสิทธิภาพของเชื้อปฏิปักษ์เห็ดนางรมฮังการี (Pleurotus ostreatus) ที่เลี้ยงในสภาพกึ่งปลอดเชื้อต่อการควบคุมไส้เดือนฝอยรากปม (Meloidogyne incognita) ของมะเขือเทศ. วารสารวิทยาศาสตร์และเทคโนโลยี 2(2): 64-72.
Adenipekun, C.O. and J.S. Gbolagade. 2006. Nutritional requirements of Pleurotus florida (Mont.) Singer, a Nigerian mushroom. Pakistan Journal of Nutrition 5(6): 597-600.
Alasri, A., M. Valverde, C. Roques, G. Michale, C. Cabassud and P. Aptel. 1993. Sporocidal properties of peracetic acid and hydrogen peroxide, alone and in combination with chlorine and formaldehyde for ultrafiltration membrane disinfection. Canadian Journal of Microbiology 39: 52-60.
Ficior, D., D. Indrea, A.S. Apahidean, M. Apahidean, R. Pop, Z. Moldovan, D. Maniutiu, R. Ganea and I. Paven. 2006. Importance of substrate disinfection on oyster mushroom (Pleurotus sp.) culture. Notulae Botanicae Horti Agrobotanici Cluj Napoca 34(1): 48-53.
Gerrits, J.P.G. 1977. The significance of gypsum applied to mushroom compost, in particular in relation to the ammonia content. Neth. Thai Journal of Agricultural Science 25(4): 288- 302.
Houdeau, G., J.M. Olivier, S. Libmond and H. Bawadikji. 1991. Improvement of Pleurotus cultivation. Mushrooms Science 13: 549-554.
Manjunathan, J. and V. Kaviyarasan. 2010. Studies on the growth requirements of Lentinus tuberregium (Fr.), an edible mushroom. Middle-East. Journal of Scientific Research in Science 5(2): 81-85.
Mejía, S.J. and E. Albertó. 2013. Heat treatment of wheat straw by immersion in hot water decreases mushroom yield in Pleurotus ostreatus. Revista Iberoamericana de Micología 30(2): 125-129.
Saritha, B. and M. Pandey. 2010. Evaluation of alternate substrate pasteurization techniques for culinary-medicinal white oyster mushroom, Pleurotus ostreatus var. florida (Agaricomycetideae) cultivation. International Journal of Medicinal Mushrooms 12(3): 309- 316.
Tesfaw, A., A. Tadess and G. Kiros. 2015. Optimum of oyster mushroom (Pleurotus ostreatus) cultivation using locally available substrates and material in Debre Berhan, Ethiopia. Journal of Applied Biology and Biotechnology 3(1): 15-20.
Wayne, R.R. 2001. Growing mushrooms the easy way home mushroom cultivation with hydrogen peroxide volume I. Available: http://jontrot.free.fr/champignons/cultureeau- oxygenee-Vols1-2new.pdf (May 20, 2010).
