Evaluation of Chemical Composition and Various Solvent Extracts of Hemp Seed Strain RPF1 on its Active Compound and Antioxidant Activity
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Abstract
In the past research and development related to hemp in Thailand mainly focuses on the utilization of stem and fiber quality while there is a few research on leaves, roots and seeds. The seedsobtained from cultivation can be classified into two types: seeds that can be used for breeding or cultivation, and seeds for consumption. The consumption seed type cannot be propagated therefore it was the agricultural wasted. So, this research interest has been paid to seeds for consumption and unprofitable. The research aims were to evaluate the nutritional values and effect of extractive solvents on the extraction efficiency of antioxidants in hemp seed (Cannabis sativa L.) strain RPF1. Different solvents including distilled water, ethanol (50%) and ethanol (99.99%) were used in the Soxhlet extraction method. The results showed that moisture content, water activity (aw), protein ash fiber carbohydrate and fat of hemp seed RPF 1 were 5.81% 0.65 36.87% 5.12% 12.04% 20.99% and 24.43%, respectively. Efficacy of antioxidant extraction from hemp seeds was compared by DPPH. It was found that ethanol (99.99%) extraction provided the highest antioxidant content (80.21 mg Trolox eq./g) (p < 0.05), while the phenolic compounds of hemp seed extract with distilled water showed the highest content (87.98 mg Gallic acid eq./g). According to the results, ethanol (99.99%) has highly efficient that can be used for the extraction with high antioxidant activity in hemp seeds. On the other hand, distilled water might be alternative option for phenolic compound extraction from hemp seeds.
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