Callus Induction from in vitro Anther Culture of Andrographis paniculata (Burm.f.) Wall.ex Nees
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Abstract
In vitro anther culture of Andrographis paniculata from Prachin Buri province for callus induction was studied. The flower was harvested at the 2-6 days after the flower bud have the highest percentage of tetrad stage initiation was use for anther culture well. The anther explants cultured on NLN medium supplemented with 0 0.1 0.5 and 1.0 mg/l BAP in combination with 0 0.1 and 0.5 mg/l NAA for 8 weeks. The result showed that the most effective treatment for callus induction (8.87 percentage) was culturing on NLN medium supplemented with 0.5 mg/l NAA in combination with 1.0 mg/l BAP. The callus cultured on NLN medium supplemented with 0.5 mg/l NAA in combination with 1.0 mg/l BAP was incubated at the low temperature of 4 °C for 1 and 3 days. It was further transplanted under high temperature of 28 30 and 32 °C for 1, 2, 3 and 4 days. The result showed that the highest weight callus was incubated on low temperature of 4 °C for 24 day, and then transplanted under high temperature of 28 °C for 1 days.
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References
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