Effects of adenine sulfate and sugar type on the growth and development of shoots induction of papaya callus in vitro

Authors

  • Adisak Intanu Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang
  • Piengpim Chidburee Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang
  • Aphichat Chidburee Agricultural Technology Research Institute, Rajamangala University of Technology Lanna, Lampang

Keywords:

Adenine sulfate, sugarcane, Callus, Papaya and In vitro

Abstract

The aim of this research work was to study the different concentrations of adenine sulfate (Ads) and sugar types on papaya's growth and development of shoot-induced callus explants. There were two experiments. The first experiment studied different Ads concentrations: no fill (control), 25, 50 and 75 mg/l. Ads was added to the Murashige and Skoog (MS) medium. Data was recorded for four weeks after culture. The results showed that the callus explants cultured on MS medium added with 50 mg/l Ads have the highest number of new shoots per explant (7.00 shoots per explant) and shoot length (5.15 mm). The second experiment examined two types of sugars, sucrose and glucose, compared with no sugar added (control). The result showed that the percentage of new shoots and the number of new shoots per explant were not different when cultured on both sugar-added media but were different from no sugar added (control). The callus explant cultured on 3% glucose added has an increased number of new shoots per explant.

References

Office of Agricultural Economics. Exports of agricultural products and products of Thailand. In the report of Thai agricultural products trade with foreign countries for the year 2021. Office of Agricultural Economics 2022;1-75.

Yingyong P. Plant Propagation. 1thed. Bangkok: Baanlaesuan Publishing; 2019.

Penkhae R, Kiriya S and Kanjana L. Propagation of papaya (Carica papaya L.) cv. Holland in tissue culture. Khon Kaen AGR. J 2019;47(3):459-66.

Petcharat C. Plant tissue culture technology for agriculture. Bangkok: Bangkok Thonburi University;2023.

Phongyuth N, Aphichat C and Pitak P. Plant Propagation by Plant Tissue Culture Technique. Rajamangala Institute of Technology 2019;40 p.

Noguchi L, Uscharee R, Somkiat S and Somchai W. Effect of disinfectants and plant growth regulator in aquatic plant Bucephalandra sp. micropropagation. King Mongkut's Agricultural Journal 2017;35:95-103.

Afshan N, Anwar S, and Mohammad A. Effect of adenine sulphate interaction on growth and development of shoot regeneration and inhibition of shoot tip necrosis under In Vitro condition in adult Syzygium cumini L.—a multipurpose tree. Appl Biochem Biotechnol 2014;173:90–102.

Nancy J, Yashodhara V and Pragati M. Elicitation enhanced the production of bioactive compound and biomass accumulation in callus cultures of Glycyrrhiza glabra L. In Vitro. Cellular and Developmental Biology – Plant 2021;58:427–436.

Badou BT, Agbidinoukoun A, Cacai GTH, Dossoukpevi RC and Ahanhanzo C. Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus). Int. J. Pure, Appl, Biosci 2017;9:1-15.

Siriwan B, Vanida C, and Supat A. Effects of plant growth regulators on papayas (Carica papaya) cultured in vitro. Kasetsart Journal (Natural Sciences) (Thailand) 1988;22(5):1-6.

Bonga JM, and Aderkas P. In vitro culture of trees. Dordrecht. Kluwer Academic Publishers, Chicago;1992;236 p.

Akhtar A, Zink D and Becker PB. Chromodomains are protein-RNA interaction modules. Nature 2000;407(6802):405-409.

Yudkin J, Edelman J and Hough L. Sugar: Chemical, Biological and Nutritional Aspects of Sucrose, Butterworth 1973;ISBN 978-0-408-70172-3.

Ponomarev VV, and Migarskaya LB. Heats of combustion of some amino-acids. Russ. J. Phys, Chem, (Engl. Transl.) 1960;34:1182–3.

Boerio-Goates J. Heat-capacity measurements and thermodynamic functions of crystalline α-D-glucose at temperatures from 10K to 340K. J. Chem, Thermodynam.1991;23(5):403–9.

Biondi S and Thorpe TA. Clonal propagation of forest tree species. In: Rao, A.N. (ed.): Proceedings COSTED Symposium on Tissue Culture of Economically Important Plants. ANBS, National University, Singapore 1981;pp.197-204.

Moran R. Formulae for determination of chlorophyllous pigments extracted with. N,N-dimethylformamide. Journal of Plant Physiology 1982;69:1376-81.

Sermsiri C, Yaowaphan S, Prakay O and Sontichai C. Shoots induction from in vitro node of teak. King Mongkut's Agricultural Journal 2018;36(2):126-34.

Namita V, Sarita A and Arya ID. Rapid and mass propagation of the economically important desert plant capparis decidua for its afforestation program. Journal of Arid Land Studies 2014;24-1:33-6.

Priyanka S, Swati C, Anita RG, Poonam D, Jyoti R, Kavita S, et al. Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco). African Journal of Biotechnology Vol 2012;11(92):15852-62.

Umnouysin S, Thepsithar C and Obsuwan K. The comparative study on an appropriate medium for in vitro growth of cassava (Manihot esculenta Cratz). Agricultural Sci. J 2009;40(1):169-72.

Somboon T. Plant physiology. green fence publishing house, Bangkok;1995.206 p.

Murashige T. Plant propagation through tissue culture. Annu. Rev, Plant Physiol 1974;25:135–66.

Waraporn C. Plant growth regulators. Faculty of Science and Technology Nakhon Sawan Rajabhat University;2010.147 p.

Siwapong C. Plant tissue culture. Faculty of Science and Technology Rajabhat Institute Udon Thani, Udon Thani;2003.

Mohanty S, Panda MK, Subudhi E and Nayak S. Plant regeneration from callus culture of curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis. Biol, Plant 2008;52:783-6.

Jala A. Effects of NAA, BA and sucrose on shoot induction and rapid micro-propagation by trimming shoot of Curcuma longa L. Amer. Trans, Eng, Appl, Sci 2011;3(2):101-9.

Vu JCV, Niedz RP and Yelenosky G. Glycerol stimulation of chlorophyll synthesis, embryogenesis andcarboxylation and sucrose metabolism enzymes in nucellar callus of “Hamlin” sweet orange. Plant Cell Tissue Org, Cult 1993;33:75-80.

ลักษณะการเจริญ และพัฒนาการของยอดใหม่จากชิ้นส่วนแคลลัสมะละกอที่เพาะเลี้ยงบนอาหาร ที่เติมน้ำตาลชนิดต่าง เป็นเวลา 4 สัปดาห์ (แถบ = 1 เซนติเมตร)

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Published

2023-08-27

How to Cite

1.
Intanu A, Chidburee P, Chidburee A. Effects of adenine sulfate and sugar type on the growth and development of shoots induction of papaya callus in vitro. Health Sci Tech Rev [Internet]. 2023 Aug. 27 [cited 2024 May 16];16(2):67-79. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/256560

Issue

Vol. 16 No. 2 (2023): May - August

Section

Research articles

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