Effects of adenine sulfate and sugar type on the growth and development of shoots induction of papaya callus in vitro

Authors

  • Adisak Intanu Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang
  • Piengpim Chidburee Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang
  • Aphichat Chidburee Agricultural Technology Research Institute, Rajamangala University of Technology Lanna, Lampang

Keywords:

Adenine sulfate, sugarcane, Callus, Papaya and In vitro

Abstract

The aim of this research work was to study the different concentrations of adenine sulfate (Ads) and sugar types on papaya's growth and development of shoot-induced callus explants. There were two experiments. The first experiment studied different Ads concentrations: no fill (control), 25, 50 and 75 mg/l. Ads was added to the Murashige and Skoog (MS) medium. Data was recorded for four weeks after culture. The results showed that the callus explants cultured on MS medium added with 50 mg/l Ads have the highest number of new shoots per explant (7.00 shoots per explant) and shoot length (5.15 mm). The second experiment examined two types of sugars, sucrose and glucose, compared with no sugar added (control). The result showed that the percentage of new shoots and the number of new shoots per explant were not different when cultured on both sugar-added media but were different from no sugar added (control). The callus explant cultured on 3% glucose added has an increased number of new shoots per explant.

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ลักษณะการเจริญ และพัฒนาการของยอดใหม่จากชิ้นส่วนแคลลัสมะละกอที่เพาะเลี้ยงบนอาหาร ที่เติมน้ำตาลชนิดต่าง เป็นเวลา 4 สัปดาห์ (แถบ = 1 เซนติเมตร)

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Published

2023-08-27

How to Cite

1.
Intanu A, Chidburee P, Chidburee A. Effects of adenine sulfate and sugar type on the growth and development of shoots induction of papaya callus in vitro. Health Sci Tech Rev [Internet]. 2023 Aug. 27 [cited 2024 Nov. 1];16(2):67-79. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/256560

Issue

Vol. 16 No. 2 (2023): May - August

Section

Research articles

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