Development of sugars quantification method using polarimeter couple with UV-Visible quartz cuvette cell

บทคัดย่อ

The objective of this study was to develop the method for determination of sugars at low sample volume using UV-Visible quartz cuvette cuvett instead of polarimeter cell. This proposed method could be analysis of sugars with high sample throughput rate and appropriate for analysis of pharmaceutical formulation. This study, the simple determination method by using sample UV-Visible quartz cuvette was developed in order to analyze dextrose, sucrose and lactose. It was found that quartz cuvette was not affected from the sodium light source at wavelength of 589.0 nm and it was possible to adapt for polarimetry technique. The linear calibration curve of dextrose, sucrose and lactose standard solution using polarimeter sample tube were constructed in the concentration ranges of 2.5–15.0 % w/v.  Linear regression between the angle of rotation (y) and concentration of each sample (x) were expressed as the equation of y = 1.0941x – 0.1824 (r²= 0.9993), y = 1.3515x –0.1581 (r²= 0.9998) and y= 1.0390x – 0.0162 (r²= 0.9997) for dextrose, sucrose and lactose, respectively. Interestingly, the calibration curve of sugars standard solution using UV-Visible quartz cuvette were shown as y = 0.1050x - 0.1038 (r²= 0.9990), y = 0.1312x – 0.1458 (r²= 0.9989) and y = 0.1072x – 0.0335 (r²= 0.9991) for dextrose, sucrose and lactose, respectively. The detection limits (LOD) of proposed method for determination of dextrose, sucrose and lactose were 0.5, 1.0 and 0.6 % w/v, respectively, while the quantitation limits (LOQ) were 1.6, 3.0 and 1.9 %w/v for anlysis of dextrose, sucrose and lactose, respectively. The interferences study for proposed method from sodium chloride was investigated. It had found that this salt shown none effect for the proposed method. The results acquired the proposed method were compared favorably with those acquired by the reference method at a 95% confidence level with no significant difference (n=10). The developed method had shown accurate, precise, and reproducible. Moreover, this method indicated the possibility of a modified polarimetric method for the quantification of sugars. The method is possible to apply both in simple quality control laboratory and local industrial that involved with pharmaceutical production.