Effect of dietary gamma-Aminobutyric acid supplementation on white leg shrimp (Litopenaeus vannamei) growth and postlarvae quality
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Abstract
The study aimed to investigate the effects of 0%, 0.2%, and 0.4% gamma-aminobutyric acid supplementation in white leg shrimp (Litopenaeus vannamei) diet on growth performance and postlarval quality follow shrimp biotech methods, molecular biology techniques, and wet mount under a microscope. The postlarvae stage PL9-PL25 during culture was investigated. The results found that gamma-aminobutyric acids extracted from plants (Hawthorn, valerian, hops, plants of the cannabis family, mint, and lemon balm) had antioxidant properties and were resistant to Vibrio parahaemolyticus EMS bacteria at a concentration of 50 mg/ml. The PL25 shrimp fed dietary gamma-aminobutyric acid supplementation 0.4% had the highest growth performance. It was 15.75±0.95 mm in total length and 0.028±0.000 g in body weight with a quality score of 95.67±1.15% and statistically significant differences (p<0.05) from the control group (0%). The survival rate was not significantly different (p>0.05), which was in the range of 89.96-93.91%. Furthermore, under salinity and formalin stress condition, the survival rate was in the same range of 98.33-99.67% (p>0.05). In all experimental groups, there was no infection by a pathogen causing early mortality or white spot disease. On the contrary, an EHP infection was found in the hepatopancreas of all experimental groups. Therefore, shrimp diets supplemented with 0.4% gamma-aminobutyric acid could improve postlarvae growth performance and shrimp quality including resistant to environmental stress conditions. The quality of white leg shrimp is still over 90% even under EHP infection conditions.
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Abdel-Naime, W. A., Fahim, J. R., Abdelmohsen, U. R., Fouad, M. A., Al-Footy, K. O., Abdel-Lateff, A. A., & Kamel, M. S. (2019). New antimicrobial triterpene glycosides from lemon balm (Melissa officinalis). South African Journal of Botany, 125, 161-167. doi: 10.1016/j.sajb.2019.07.004
Bae, J., Hamidoghli, A., Farris, N. W., Olowe, O. S., Choi, W., Lee, S., Won, S., Ohh, M., Lee, S., & Bai, S. C. (2022). Dietary γ-aminobutyric acid (GABA) promotes growth and resistance to Vibrio alginolyticus in Whiteleg shrimp (Litopenaeus vannamei). Aquaculture Nutrition. doi: 10.1155/ 2022/9105068
Bahorun, T., Gressier, B., Trotin, F., Brunet, C., Dine, T., Luyckx, M., Vasseur, J., Cazin, M., Cazin, J. C., & Pinkas, M. (1996). Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-forschung, 46(11), 1086-1089.
Bahri, H., Benkirane, C., & Tazi, B. (2019). Antioxidant activity of hawthorn (Crataegus monogyna) from Morocco. Academia Journal of Medicinal Plants, 7(2), 030-035. doi: 10.15413/ajmp.2019.0100
Boonyawiwat, V., Patanasatienkul, T., Kasornchandra, J., Poolkhet, C., Yaemkasem, S., Hammell, L., & Davidson, J. (2017). Impact of farm management on expression of early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) on penaeid shrimp farms in Thailand. Journal of Fish Diseases, 40(5), 649-659. doi: 10.1111/jfd.12545
Boonta, T., Chitmanat, C., & Promya, J. (2012). Effects of Spirulina platensis, Cladophora sp. and Allium sativum supplementary diets on growth performance, reproductive maturity, and phagocytic activity in common lowland frog (Rana rugulosa). Journal of Fisheries Technology Research, 6(1), 23-35. (in Thai)
Chang, Y. P., Liu, C. H., Wu, C. C., Chiang, C. M., Lian, J. L., & Hsieh, S. L. (2012). Dietary administration of zingerone to enhance growth, non-specific immune response, and resistance to Vibrio alginolyticus in Pacific white shrimp (Litopenaeus vannamei) juveniles. Fish & Shellfish Immunology, 32(2), 284-290.
doi: 10.1016/j.fsi. 2011.11.017
Charoendat, U., Koedprang, W., & Thawonsuwan, J. (2021). Effects of crude extract of Hazardana (Phyllanthus urinaria) on hepatopancreatic health status, antioxidant, immune response and resistance to acute hepatopancreatic necrosis disease in Pacific white shrimp (Litopenaeus vannamei). RMUTSV Research Journal, 13(3), 717-731. (in Thai)
Cherubini, E., Gaiarsa, J. L., & Ben-Ari, Y. (1991). GABA: an excitatory transmitter in early postnatal life. Trends in Neurosciences, 14(12), 515-519. doi:10.1016/0166-2236(91)90003-d.
Chirawithayaboon, P., Areechon, N., & Meunpol, O. (2020). Hepatopancreatic antioxidant enzyme activities and disease resistance of Pacific white shrimp (Litopenaeus vannamei) fed diet supplemented with garlic (Allium sativum) extract. Agriculture and Natural Resources, 54(4), 377-386.
Chuchird, N., Munkongwongsiri, N., Taemmesub, T., Tongsri, P., Somboon, M., Limsuwan, C., Sritunyalucksana, K., & Flegel, T. W. (2016). The dark threat microsporidian Enterocytozoon hepatopenaei (EHP) in Pacific white shrimp. Thai shrimp Newspaper, 13(171), 95-106. (in Thai)
Department of Fisheries. (2019). Guidelines and practices for the prevention and control of white spot disease in marine shrimp. Accessed March 4, 2023. Retrieved from https://www4.fisheries.go.th/ local/file_document/20190220224758_1_file.pdf (in Thai)
Department of Fisheries. (2018). Standard criteria for evaluation of white leg shrimp postlarvae. Accessed March 4, 2023. Retrieved from https://www4.fisheries.go.th/local/file_document/ 20190301135305_1_file.pdf (in Thai)
Dinis, T. C., Maderia, V. M. C, & Almeida, L. M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169. doi: 10.1006/abbi. 1994.1485
Direkbusarakom, S., Herunsalee, A., Yoshimizu, M., & Ezura, Y. (1996). Antiviral activity of several Thai traditional herb extracts against fish pathogenic viruses. Fish Pathology, 31(4), 209-213.
Farris, S., Unalan, I. U., Introzzi, L., Fuentes-Alventosa, J. M., & Cozzolino, C. A. (2014). Pullulan-based films and coatings for food packaging: Present applications, emerging opportunities, and future challenges. Journal of Applied Polymer Science, 131(13). doi: 10.1002/app.40539
Froehlicher, T., Hennebelle, T., Martin-Nizard, F., Cleenewerck, P., Hilbert, J. L., Trotin, F., & Grec, S. (2009). Phenolic profiles and antioxidative effects of hawthorn cell suspensions, fresh fruits, and medicinal dried parts. Food Chemistry, 115(3), 897-903. doi: 10.1016/j.foodchem.2009.01.004
Gonelimali, F. D., Lin, J., Miao, W., Xuan, J., Charles, F., Chen, M., & Hatab, S. R. (2018). Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Frontiers in Microbiology, 9, 1639. doi: 10.3389/fmicb.2018.01639
Jannoey, P., Niamsup, H., & Lumyong, S. (2010). Comparison of γ-aminobutyric acid production in Thai rice grains. World Journal of Microbiology and Biotechnology, 26, 257-263. doi: 10.1007/s11274-009-0168-2
Jorgensen, E. M. (2005). GABA. Accessed March 16, 2023. Retrieved from http://www.wormbook.org/chapters/www_gaba/gaba.html
Kamble, M. T., Rudtanatip, T., Soowannayan, C., Nambunruang, B., Medhe, S. V., & Wongprasert, K. (2022). Depolymerized fractions of sulfated galactans extracted from Gracilaria fisheri and their antibacterial activity against Vibrio parahaemolyticus and Vibrio harveyi. Marine Drugs, 20(8), 469. doi.org/10.3390/md20080469
Keawtawee, T., Suksri, A., & Denpratya, N. (2019). Effects of Spirulina platensis supplemented diets on growth performance and resistance against Vibrio parahaemolyticus in white shrimp (Litopenaeus vannamei Boone, 1931). Khon Kaen Agriculture Journal, 47(Suppl. 2), 515-520. (in Thai)
Keser, S., Celik, S., Turkoglu, S., Yilmaz, O., & Turkoglu, I. (2014). The investigation of some bioactive compounds and antioxidant properties of hawthorn (Crataegus monogyna subsp monogyna Jacq). Journal of Intercultural Ethnopharmacology, 3(2), 51-55. doi: 10.5455/jice.20140120103320
Khan, A. W., Hossain, S. S., & Uddin, S. N. (2007). Isolation, identification and determination of antibiotic susceptibility of Vibrio parahaemolyticus from shrimp at Khulna region of Bangladesh. Research Journal of Microbiology, 2(3), 216-227. doi:10.3923/jm.2007.216.227
Kostic, D., Velickovic, J. M., Mitic, S. S., Mitic, M., & Randelovic, S. S. (2012). Phenolic content, and antioxidant and antimicrobial activities of Crataegus Oxyacantha L (Rosaceae) fruit extract from Southeast Serbia. Tropical Journal of Pharmaceutical Research, 11(1), 117-124. doi: 10.4314/tjpr.v11i1.15
Liang, Y., Wu, Y., Li, J., Peng, R., Jiang, M., Jiang, X., Chen, S., & Lin, J. (2022). Effects of ammonia toxicity on the histopathology, detoxification, oxidative stress, and immune response of the cuttlefish Sepia pharaonis and the mitigation of γ-aminobutyric acid. Ecotoxicology and Environmental Safety, 232, 113256. doi: 10.1016/j.ecoenv.2022.113256
Liao, M., Wang, F., Huang, L., Liu, C., Dong, W., Zhuang, X., Yin, X., Liu, Y., & Wang, W. (2023). Effects of dietary Ginkgo biloba leaf extract on growth performance, immunity and environmental stress tolerance of Penaeus vannamei. Fish & Shellfish Iimmunology, 132, 108500. doi.org/10.1016/j.fsi.2022. 108500
Liu, B., Ge, X., He, Y., Xie, J., He, Y., Zhou, Q., Pan, L, & Chen, R. (2010). Effects of anthraquinones extracted from Rheum officinale Bail on the growth, non-specific immune response of Macrobrachium rosenbergii. Aquaculture, 310(1-2), 13-19. doi: 10.1016/j.aquaculture.2010.09.020
Lyu, J. I., Ryu, J., Seo, K. S., Kang, K. Y., Park, S. H., Ha, T. H., Ahn, J. W., & Kang, S. Y. (2022). Comparative study on phenolic compounds and antioxidant activities of Hop (Humulus lupulus & nbsp;L.) strobile extracts. Plants (basel), 11(1), 135. doi: 10.3390/plants11010135
Manok, S., & Limcharoen, P. (2015). Investigating antioxidant activity by DPPH, ABTS and FRAP assay and total phenolic compounds of herbal extracts in Ya-hom thepphachit. Advanced Science, 15(1), 106-117. (in Thai)
Munkongwongsiri, N., Limsuwan, C., & Chuchird, N. (2013). Effects of postlarval quality on occurrence of early mortality syndrome in Litopenaeus vannamei culture ponds. Proceedings of the 51st Kasetsart University annual conference (pp. 174-181). Bangkok: Kasetsart University. (in Thai)
Ngo, D.-H., & Vo, T. S. (2019). An updated review on pharmaceutical properties of γ-aminobutyric acid. Molecules, 24(15), 2678. doi: 10.3390/molecules24152678
Onsanit, S., Thitithanakul, S., Seubsang, C., Julpon, B., Timthong, A., & Tidkongrach, S. (2020). Pigment accumulation patterns, immune systems and antioxidant activities by anthocyanins from dry ripe malabar fruit (Melastoma malabathricum) in banana shrimp (Penaeus merguiensis) feed. Khon Kaen Agriculture Journal, 48(Suppl. 1), 967-974. (in Thai)
Pawana, A., Laohavisuti, N., & Wangwibulkit, S. (2017). Effect of using Suaeda maritima leave extract as a feed additive on the resistance to Vibrio parahaemolyticus and the growth of Pacific white shrimp (Litopenaeus vannamei) during nursing. King Mongkut’s Agricultural Journal, 35(3), 36-41. (in Thai)
Penprapai, N., Penprapai, P., Ratanamusik, A., & Chumchareon, M. (2017). Effect of coconut oil with extracted Curcuma zedoaria supplement in diet on growth, oxidative defense and immune system of Pacific white shrimp (Litopenaeus vannamei). (Research report). Songkhla. Faculty of Agriculture, Rajamangala University of Technology Srivijaya. (in Thai)
Proestos, C., & Komaitis, M. (2009). Antioxidant capacity of Hops. In V. R. Preedy (Ed.),
Beer in health and disease prevention (pp. 467-474). United States: Elsevier Inc. Academic Press doi: 10.1016/B978-0-12-373891-2.00045-6
Puycha, K., & Tanee, S. (2014). Effect of vitamin C on growth and health in aquatic animals. Rajabhat Agriculture Journal, 13(2), 63-70. (in Thai)
Ruchirawat, K., & Vaikrudha, K. (2003). Quality inspection of shrimp fry using the “Biotech Lab” method. In S. Dilokkiat (Ed.), Thai shrimp, a new step, one of the sustainable world leaders (pp. 271-280). Bangkok, Thailand: K. Pon printing (in Thai)
Rudtanatip T., Lynch, S. A., Culloty, S. C., & Wongprasert, K. (2018). Sulfated galactans from red algae Gracilaria fisheri increase haemocyte proliferation in common prawn Palaemon serratus. Chulalongkorn Medical Journal, 62(3), 293-295. (in Thai)
Songkhla Aquatic Animal Health Research Center. (2022). EHP management in marine shrimp hatcheries and nurseries. Accessed March 4, 2023. Retrieved from https://www4.fisheries.go.th/dof/wellwishes. (in Thai)
Songkhla Aquatic Animal Health Research Center. (2018). Manual for aquatic animal’s disease diagnosis using PCR techniques. Songkhla: Songkhla Aquatic Animal Health Research Center, Department of Fisheries. (in Thai)
Srimoon, R., & Niyomwan, S. (2021). Development and validation of the Folin-Ciocalteu semi-micro method for total phenolic compounds determination in Rangdaeng (Ventilago denticulata willd.) extract. Burapha Science Journal, 26(1), 378-398. (in Thai)
Srithaworn, M., Thuyhun, A., Chunhachart, O., & Preecharram, S. (2015). Antioxidant and antibacterial activities of crude extracts from Ya-Keaw formula against shrimp pathogens. SDU Research Journal Science and Technology, 8(2), 117-132.
Tadic, V. M., Dobric, S., Markovic, G. M., Dordevic, S. M., Arsic, I. A., Menkovic, N. R., & Stevic, T. (2008). Anti-inflammatory, gastro protective, free-radical-scavenging, and antimicrobial activities of hawthorn berries ethanol extract. Journal of Agricultural and Food Chemistry, 56(17), 7700-7709. doi.org/10.1021/jf801668c
Temu, V., Kim, H., Hamidoghli, A., Park, M., Won, S., Oh, M., Han, J. K., & Bai, S. C. (2019). Effects of dietary γ -aminobutyric acid in juvenile Nile tilapia (Orechromis niloticus). Aquaculture, 507(5), 475-480. doi: 10.1016/j.aqua culture. 2019.04.063
Thawonsuwan, J., Kongkumnerd, J., Thavonyuthikarn, M., & Kasornchandra, J. (2010). Efficacy of oil macerated garlic extract on immune responses and diseases resistance in White shrimp (Litopeneaus vannamei Boone). Proceedings of the 7th National symposium on marine shrimp (pp. 91-102). Bangkok: National Center for Genetic Engineering and Biotechnology (BIOTEC). (in Thai)
Tummarongkongsatit, A. (2007). Effective of Thai herb extracts to inhibit bacterial pathogens in Giant freshwater prawn (Macrobrachium rosenbergii). (Master’s thesis). Chiang Mai. Maejo University. (in Thai)
Van den Pol, A. N. (2003). Weighing the role of hypothalamic feeding neurotransmitters. Neuron, 40(6), 1059-1061. doi: 10.1016/s0896-6273(03)00809-2
Wu, F., Liu, M., Chen, C., Chen, J., & Tan, Q. (2016). Effects of dietary γ aminobutyric acid on growth performance, antioxidant status, and feeding-related gene expression of juvenile grass carp, Ctenopharyngodon idellus. Journal of the World Aquaculture Society, 47(6), 820-829. doi: 10.1111/jwas.12327
Xie, S. W., Li, Y. T., Zhou, W. W., Tian, L. X., Li, Y. M., Zeng, S. L., & Liu, Y. J. (2017). Effect of γ-aminobutyric acid supplementation on growth performance, endocrine hormone and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei, fed low fishmeal diet. Aquaculture Nutrition, 23(1), 54-62. doi: 10.1111/anu.12358
Yin, X., Zhuang, X., Luo, W., Liao, M., Huang, L., Cui, Q., Huang, J., Yan, C., Jiang, Z., Liu, Y., & Wang, W. (2022). Andrographolide promote the growth and immunity of Litopenaeus vannamei, and protects shrimps against Vibrio alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway. Frontiers in Immunology, 13, 990297. doi: 10.3389/fimmu.2022.990297
Yu, H., Pei, J., Qiu, W., Mei, J., & Xie, J. (2022). The antimicrobial effect of Melissa officinalis L. essential oil on Vibrio parahaemolyticus: Insights based on the cell membrane and external structure. Frontiers in Microbiology, 13, 812792. doi: 10.3389/fmicb.2022.812792
Zhang, C., Wang, X., Wang, C., Song, Y., Pan, J., Shi, Q., Qin, J., & Chen, L. (2021). Γ-aminobutyric acid regulates glucose homeostasis and enhances the hepatopancreas health of juvenile Chinese mitten crab (Eriocheir sinensis) under fasting stress. General and Comparative Endocrinology, 303(2):113704. doi: 10.1016/j.ygcen.2020.113704
