Dietary tamarind (Tamarindus indica) seed husk supplementation in rooster on reproductive performances and cool storage semen
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Abstract
The objective of this study was to determine the effects of dietary tamarind seed husk (TSH) supplementation on rooster semen quality. TSH was extracted using a spray dryer. The phenolic and total phenolic contents were analyzed and found to be 7435.08 mg/g DM and 503.95 mg/g DM, respectively. Antioxidant activity was assessed using various assays, including radical scavenging capacity, ferric reducing antioxidant power (FRAP) assay (3538.56 μM FeSO₄/ml), 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay (85.70%), and Trolox equivalent antioxidant capacity (TEAC) assay (69.81%), indicating that TSH possesses high antioxidant activity. The experiment followed a randomized complete block design (RCBD). Twenty-four Thai native roosters (1–1.5 years old) were divided into four groups. The four treatments included dietary TSH supplementation at 0, 0.3, 0.6, and 0.9 mg/kg. Semen was collected twice per week for six weeks. Different storage temperatures (5 °C and 25 °C) and durations (0, 12, and 24 hours) were used to evaluate semen quality and determine the optimal storage conditions. Semen quality evaluation included motion parameters, progressive motility, and viability. The results indicated that total motility, progressive motility, and the percentage of viable sperm in the 0.6 mg/kg TSH group were significantly higher than in the other groups (p< 0.05). These findings suggest that dietary supplementation with 0.6 mg/kg TSH improves the quality of fresh semen, particularly in terms of motion parameters and sperm viability, making it suitable for semen storage or artificial insemination in roosters.
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