Effect of Water Extraction Temperature on the Efficiency of α-Glucosidase Inhibition by Flavonoids from Dried Cannabis Leaves
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Abstract
This study aimed to evaluate the α-glucosidase inhibitory activity of flavonoids extracted with water from dried cannabis leaves. Flavonoid extraction was performed using water at 40°C, 80°C, and 100°C. The flavonoid content was analyzed using the Aluminum Chloride Colorimetric Assay, while α-glucosidase inhibitory activity was assessed using the Dinitrosalicylic Acid (DNS) Assay. The results indicated that extraction at 100°C yielded the highest flavonoid content (1.12 mg QE/g extract). The extracted flavonoids were identified as water-soluble flavonoid glycosides that demonstrated significant α-glucosidase inhibitory potential. The IC50 value of the flavonoid extract was determined to be 97.36 µg/mL, compared to 14.81 µg/mL for acarbose. The results confirmed that higher water temperatures significantly enhance the dissolution and release of flavonoids. This study highlights the potential of developing cannabis leaf tea products to help reduce blood sugar levels, which could be beneficial for managing type 2 diabetes. Furthermore, the findings support the sustainable use of medicinal plants in traditional and alternative Thai medicine.
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