ATR-FTIR and GC-MS Methods for Detection of Sibutramine Adulterated in Slimming Products of Instant Coffee
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Abstract
One of the most common adulterants: Sibutramine has been found in slimming products. This work aimed to study the application of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and gas chromatography coupled with mass spectrometry (GC-MS) to detect sibutramine in slimming products in the form of Instant coffee. Twenty instant coffee products were acquired from local markets and online stores. At wavenumber 4000-650 cm-1, ATR-FTIR analysis was performed. The mass spectra of the sample indicate a distinct peak for sibutramine. The screening test obtained by ATR-FTIR could be confirmed by GC-MS. The screening was performed on selected ion monitoring mode at 58, 72, and 114 amu (Sibutramine) at a retention time 23.997 minute. Furthermore, laxatives (Bisacodyl) were detected in samples evaluated by GC-MS at a retention time 36.534 minute, while antibiotic (Paromomycin) were detected at a retention time 6.089 minute. This is qualitative investigation using ATR-FTIR and GC-MS to discover sibutramine adulterated in instant coffee slimming products. Both techniques are capable of detecting sibutramine in slimming Instant coffee, which could be useful in forensic examination.
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