Effect of Plant Growth Regulators on Micropropagation of Nymphaea ‘Aurora’
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Abstract
Water-lilies (Nymphaea spp.) are among the most popular aquatic plants due to their beautiful flowers, making them highly valued. The Nymphaea ‘Aurora’ is a hybrid hardy Water-lilies with small flowers that bloom profusely. Plant tissue culture technology has been employed in propagating plants to achieve desired characteristics and rapidly increase their numbers. This research aims to investigate appropriate sterilization methods for the rhizomes and young shoots. It also examines the effects of growth regulators on root induction and callus induction in aseptic conditions using MS (Murashige and Skoog, 1962) medium by semi-solid and liquid with temporary immersion bioreactor systems (TIBs). It was found that the most effective method for sterilizing the rhizome explants involved using 10 percent sodium hypochlorite solution for 20 minutes, followed by 0.2 percent mercuric chloride solution for 10 minutes. This method achieved a 100 percent sterility rate and an 86.67 percent survival rate. The shoots from the rhizome parts were able to induce shoots and leaves better than the young shoots. Tissue culture of the dwarf Water-lilies in liquid MS medium supplemented with 10 µM BAP (6-Benzylaminopurine) and 5 µM IAA (Indole-3-acetic acid) using a temporary immersion bioreactor system showed that shoot induction was higher than on semi-solid medium. This method produced the highest number of shoots and leaves, with 1.78 shoots and 14.00 leaves within six weeks. Regarding the effect of growth regulators on root induction, MS medium supplemented with 10 µM NAA (Naphthaleneacetic acid) induced the highest number of roots, with 16.22 roots. Concerning the effect of growth regulators on callus induction in young leaf, young shoot, and root cultures on semi-solid MS medium supplemented with 6 µM TDZ (Thidiazuron) and 0.5 µM NAA, it was found that the leaf and root tissues enlarged but did not develop into callus.
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