Genetic variation of Mystus nemurus (Cuv. & Val.) in Lam Nam Kam by Random Amplified Polymorphic DNA (RAPD) Technique
Article Sidebar
Main Article Content
Abstract
The purpose of this research was to assess genetic diversity of Yellow catfish (Mystus nemurus) (Cuv. & Val.) from five subpopulations including Sarasawadi Floodgate (A), Bang Nong Bueng Floodgate (B), Ban Na Khu Floodgate (C), Ban Nakham Floodgate (D), Lower Nam Kam Floodgate and flowing down to Mekong River at That Phanom district, Nakhon Phanom province (E). The genetic variation was done with nine primers (OPB-07, OPB-10, OPC-02, OPC-05, OPC-11, OPC-13, OPC-15, OPC-16 and UBC122). The size was between 200 – 2,500 and the total number of 97 DNA bands of 95 bands were found to be percent of polymorphic band between 46.39–81.44. The gene diversity (H) value was between 0.18–0.32. The value of gene differentiation (GST) and value of gene flow (Nm) overall populations in this study was 0.29 and 1.22, respectively. The genetic distance (D) values were found between 0.132-0.341. The UPGMA (Unweighted pair group method with arithmetic means) dendrogram clearly separated yellow catfish (Mystus nemurus) from Lum Nam Kam into two groups: the first group consisted of Sarasawadi Floodgate (A) and the second group consisted of Bang Nong Bueng Floodgate (B), Bang Na Khu Floodgate (C), Bang Nakham Floodgate (D) and Lower Num Khum Floodgate, flowing down to Mekong River at That Phanom district, Nakhon Phanom province (E). This research was very useful for breeding, management, and studying on the genetic structure of yellow catfish population in Lam Nam Kam in the future.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Amatyakul, C., Benjakarn, M., Sriwattana, W., Sumanochitphon, S., Sriphattharaprasit, P., Jeso, S.,Sihiranwong, A., Sihirunwong, S., Kasisuwan, S., & Leelawiwat, W. (1995). Yellow catfish. Bangkok: Inland Fisheries Division Department of Fisheries, Ministry of Agriculture and Cooperatives Publishers. (in Thai)
Bala, B., Mallik, M., Saclain, S., & Islam, M.S., (2017). Genetic variation in wild and hatchery populations of giant freshwater prawn (Macrobrachium rosenbergii) revealed by randomly amplified polymorphic DNA markers. J Genet Eng Biotechnol, 15(1), 23-30.
Hanpongkittikul A. (2017). Management of Watergate to Enhance Fisheries In the Nam Kam River System, Thailand. (Doctoral Thesis). University of Hull. England.
Kasiswan, S., Ruangkul, J., & Chesoh, S. (2000). Effect of Salinity on Growth and Survival Rate of Green Catfish, Mystus filamentus, Juvenile during Nursing Period. Pattani: Pattani Inland Fisheries Development Center Publishers. (in Thai)
Klinbunga, S., Ampayup, P., Tassanakajon, A.., Jarayabhand, P., & Yoosukh, W., (2000). Development of species-specific markers of tropical oyster (Crassostrea belcheri) in Thailand. Marine Biotechnology, 2,476-484.
Kumla, S. (2011). Select of Yellow catfish (Mystus nemurus) Broodstock by Using RAPD, ISSR and Microsatellite. (Doctoral Thesis) Khon Kaen University. Khon Kaen. (in Thai)
Kumla S., Doolgindachbaporn S., Sudmoon R., & Sattayasai N. (2012). Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (C&V) in Thailand using RAPD, ISSR and SCAR marker. Molecular Biology Report. 39, 501-510.
Kumla, S., Rayan, S., & Chartchumni, B. (2016). Genetic diversity of Yellow Catfish, Mystus nemurus (C&V), in Nam Oun Dam, Sakon Nakhon Province. Research Journal. 9 (1). (in Thai)
Leesanga, S, Siraj, S.S., Daud, S.K., Tan, S.G. & Harmin S.A. (2004). Intraspecific polymorphism in Mystus nemurus (C&V) detected by RAPD-PCR fingerprinting. Pertanika Journal Tropical Agriculture Science 27, 11-20.
Mahboob, S., Al-Ghanim, K.A., Al-Misned, F., Al-Balawi, H.F.A., Ashraf, A., & Al-Mulmim, M.A.A. (2019). Genetic diversity in tilapia populations in a freshwater reservoir assayed by randomly amplified polymorphic DNA markers. Saudi J Biol Sci, 26(2), 363-367.