Isolation and optimization of nitrate and phosphate removal by green algae isolated from wastewater of food industrial
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Abstract
Green algae are able to remove inorganic nitrogen and phosporus from wastewater. This work aimed to isolate green algae from food processing industrial wastewater in Phra Nakhon Si Ayutthaya province that has highly efficient removal of nitrate and phosphate from wastewater. It was found that twenty-one green algae strains were isolated from wastewater samples. All green algal isolates were grown in sterile wastewater (pH 7) at 30 ºC under light intensity of 30 µmolphoton/m2/s for 18 h per day and shaking at 120 rpm for 5 days before measuring chemical characteristics of wastewater after cultivation. The results revealed that isolate FPI 18 showed the highest nitrate and phosphate removal and also showed the lowest BOD and COD when compared with other green algae isolates. The genetics characterization of green algal isolate FPI 18 was classified by using 18S rDNA squence and phylogenetics tree analysis. The results indicated that green algae isolate FPI 18 showed their similarities comparing to the green alge in the genus of Tetraspora. Thereby, this strain was named Tetraspora sp. Rmutsb FPI 18. In morphological characterization under light microscope, Tetraspora sp. Rmutsb FPI 18 has an oval shape and 5-6 µm of diameter. In addition, single cells and 3-4 cells surrounded by transparent sheath can be observed. Furthermore, some parameters for nitrate and phosphate removal by this strain such as pH, temperature and light intensity were also examined. The results showed that the best condition for nitrate and phosphate removal from wastewater by Tetraspora sp. Rmutsb FPI 18 were initial pH 8, temperature at 30 ºC and light intensity of 50 µmolphoton/m2/s for 18 h per day. In addition, the reduction of nitrate and phosphate under these conditions corresponded to the increasing of Tetraspora sp. Rmutsb FPI 18 biomass. The best of each condition was combined and designated as “optimal condition”. The optimal condition in wastewater was used for cultivation of Tetraspora sp. Rmutsb FPI 18 and compared with control condition (wastewater initial pH 7, 25 ºC and 30 µmolphoton/m2/s for 18 h per day). The results revealed that Tetraspora sp. Rmutsb FPI 18 showed high capability to remove nitrate and phosphate from wastewater with removal efficiency of 93.65 and 96.65%, respectively under cultivated cell in optimal condition.
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Published manuscript are the rights of their original owners and RMUTSB Academic Journal. The manuscript content belongs to the authors' idea, it is not the opinion of the journal's committee and not the responsibility of Rajamangala University of Technology Suvarnabhumi
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