Investigation of antioxidant capacity and antioxidant activity of Ma-huad (Lepisanthes rubiginosa (Roxb.) Leenh.) juice
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Abstract
Ma-huad (Lepisanthes rubiginosa (Roxb.) Leenh.) is a perennial plant of the Sapindaceae family. This plant has been found in the sparse forest of Thailand and neighboring countries. The color of ripe Ma-huad fruits is red - purple and the active substances found in this fruit are anthocyanins. Furthermore, others polyphenols, may be found as constituents in this plant. In the present study, antioxidant contents and antioxidant activity of Ma-huad juices were determined. Preparation of Ma-huad juices was done by using the ratio of ripe Ma-huad fruit weight and distilled water into 1:1, 1:2 and 1:3, respectively. The boiling temperature was controlled at 95 oC while the boiling times were changed from 5 to 15 minutes (5-minute intervals). Some physical properties of Ma-huad juices were analyzed. These samples were determined for total anthocyanin content by using pH-differential method, total phenolic content by using Folin-Ciocalteu reagent, antioxidant activity by DPPH and ABTS assays. The results showed that the maximum total anthocyanin contents, total phenolic contents and antioxidant activities from DPPH and ABTS methods were 86.00±1.77 mg CGE/100g of FW, 118.64±1.40 mg GAE/100 g crude extract, 57.78%, and 96.62%, respectively which was obtained from T3 sample (ratio of ripe Ma-huad fruit weight and distilled water was 1:1 at 15 minutes). Total anthocyanin contents and total phenolic contents were highly correlated to the percentage of the inhibition of antioxidant activity both DPPH assay (r=0.82 and 0.88) and also ABTS assay (r=0.64 and 0.72) at 0.05 significance (p>0.05).
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Published manuscript are the rights of their original owners and RMUTSB Academic Journal. The manuscript content belongs to the authors' idea, it is not the opinion of the journal's committee and not the responsibility of Rajamangala University of Technology Suvarnabhumi
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