The effect of dietary antioxidant supplement on oxidative stress

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Wassana Intasang
Phawit Norchai
Mart Maiprasert


          Oxidative stress is a status occurred by an imbalance between production and accumulation of oxygen reactive species (ROS) in cells and tissues that lead to oxidative damage of DNA, protein, fat molecules. It increases an inflammation and chronic diseases such as non-communicable diseases (NCDs) that mainly caused by unhealthy lifestyle and environment. Recently, dietary antioxidant supplementation is one of the most comfort way to decrease free radical content and support antioxidant activity. This study aimed to evaluate the effects of antioxidants activity on supplementary diet intake. The quasi-experimental study was conducted in 20 participants. Blood samples were obtained 2 times, before and after continuously daily dietary supplement intake for 14 days. Free radicals and antioxidant content were analyzed. The demographic data showed a total of healthy participants were enrolled with mean age of 34.90±9.58 years, body weight of 57.93±11.15 kilograms and 80% were female. The results showed a significant reduction of mean difference in free radical level of P=0.04 (95% CI, 1.23-49.37) and significant increase mean difference in antioxidant content of P<0.01 (95% CI, 0.03-0.15) under the effect of dietary supplement. This dietary supplement significantly reduced free radical and increased antioxidant content that may reduce oxidative stress in healthy working-age participants with continuous daily intake. However, lifestyle modification and randomized controlled trial should be recommended for additional evidence.


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Betteridge, D. J. (2000). What is oxidative stress?. Metabolism, 49(2, Suppl 1), 3-8.

Brasnyó, P., Molnár, G. A., Mohás, M., Markó, L., Laczy, B., Cseh, J., Mikolás, E., Szijártó, I. A., Mérei, A., Halmai, R., Mészáros, L. G., Sümegi, B., & Wittmann, I. (2011) Resveratrol improves insulin sensitivity, reduces oxidative stress and activates the Akt pathway in type 2 diabetic patients. British Journal of Nutrition, 106(3), 383-389.

Brown, D. R., Gough, L. A., Deb, S. K., Sparks, S. A., & McNaughton, L. R. (2017). Astaxanthin in exercise metabolism, performance and recovery: A review. Frontiers in Nutrition, 4, 76.

Callergari1930, (2020). Health screening by CR3000. Retrieved 10 December 2020, from

Carillon, J., Knabe, L., Montalban, A., Stévant, M., Keophiphath, M., Lacan, D., ... & Rouanet, J. M. (2014). Curative diet supplementation with a melon superoxide dismutase reduces adipose tissue in obese hamsters by improving insulin sensitivity. Molecular Nutrition & Food Research, 58(4), 842-850.

Chuayjaroen, P. (2015). The effect of boiling on antioxidant activity of King Oyster Mushroom, Jew’s ear mushroom, Phoenix oyster mushroom and Shitake mushroom. The 6th Academic Meeting National and International Conference (pp. 382-390). Bangkok: Huachiew Chalermprakiet University. (in Thai)

Division of Non-Communicable Diseases, Department of Disease Control, Ministry of Public Health. (2019). Number of patients and deaths from hypertension, diabetes, coronary heart disease and cerebrovascular disease of Thai people 2016-2018. Retrieved December 10, 2019, from (in Thai)

Duangjit, S., Suwannarat, K., Kittiphinitnunta, K., Ongwisut, P., Bumrungthai, S., Ngawhirunpat, T., Charoenputtakun, P., & Sila-On, W. (2019) Role of natural antioxidants for topical applications: Properties, efficacy, safety and novel delivery systems. IJPS, 15(1), 21-48. (in Thai)

Hebbar, V., Shen, G., Hu, R., Kim, B. R., Chen, C., Korytko, P. J., Crowell, J. A., Levine, B. S., & Kong, A. N. (2005). Toxicogenomics of resveratrol in rat liver. Life Sciences, 76(20), 2299-2314.

Hernández-Camacho, J. D., Bernier, M., López-Lluch, G., & Navas, P. (2018). Coenzyme Q(10) supplementation in aging and disease. Frontiers in physiology, 9, 44.

Kishimoto, Y., Yoshida, H., & Kondo, K. (2016). Potential anti-atherosclerotic properties of astaxanthin. Marine Drugs, 14(2), 35.

Lodovici, M., Bigagli, E., Luceri, C., Manni, M., & Zaid, M. (2011). Protective effect of resveratrol against oxidation stress induced by 2-nitropropane in rat liver. Pharmacology & Pharmacy, 2(3), 127-135.

Maipasert, M. (2019). Anti-aging by Dr. Mart. Bangkok: Mart Maipasert. (in Thai)

Mamah, B., Nisan, N., Duanyai, S., & Manok, S., (2017). Development of cosmetic product from leaves of Moringa oleifera Lam. collected in Sripoom Community in Thonburi area. Isan Journal of Pharmaceutical Sciences, 2, 80-89. (in Thai)

Mukherjee, S., Dudley, J. I., & Das, D. K. (2010). Dose-dependency of resveratrol in providing health benefits. Dose-response, 8(4), 478–500.

Nishida, Y., Yamashita, E., & Miki, W. (2007). Quenching activities of common hydrophilic and lipophilic antioxidants against singlet oxygen using chemiluminescence detection system. Carotenoid Science, 11(6), 16-20.

Phunsawan, B. (2013). Free radicals, antioxidants and analysis of antioxidant activity. Journal of Science and Technology, 21(3), 275-286. (in Thai)

Rehou, S., Shahrokhi, S., Natanson, R., Stanojcic, M., & Jeschke, M. G. (2017). Antioxidant and trace element supplementation reduce the inflammatory response in critically Ill burn patients. Journal of Burn Care & Research, 39(1), 1-9.

Sakunphueak, A. (2004) Free radical and antioxidant. Retrieved 10 December 2020, from (in Thai)

Salehi, B., Mishra, A. P., Nigam, M., Sener, B., Kilic, M., Sharifi-Rad, M., Fokou, P., Martins, N., & Sharifi-Rad, J. (2018). Resveratrol: A double-edged sword in health benefits. Biomedicines, 6(3), 91.

Singsai, K., Sakdavirote, A., Wechpanishkitkul, K., & Moonsamai, A. (2020). The comparison of phenolic compounds, flavonoids and antioxidant activities of the ethanolic extracts of shoots, leaves, fruits and seeds of Leucaena leucocephala. Naresuan Phayao Journal, 13(3), 66-73. (in Thai)

Sutin, S. (2016). Vitamins and free radicals. Huachiew Chalermprakiet Science and Technology Journal, 2, 80-92. (in Thai)

Tiahou, G., Maire, B., Dupuy, A., Delage, M., Vernet, M. H., Mathieu-Daudé, J. C., . . . & Cristol, J. P. (2004). Lack of oxidative stress in a selenium deficient area in Ivory Coast. European Journal of Nutrition, 43(6), 367-374.

Tongco, M. D. C. (2007). Purposive sampling as a tool for informant selection. Ethnobotany Research & Applications, 5, 147-158.

Ullegaddi, R., Powers, H. J., & Gariballa, S. E. (2006). Antioxidant supplementation with or without B-group vitamins after acute ischemic stroke: a randomized controlled trial. JPEN Journal of parenteral and enteral nutrition, 30(2), 108–114.

Weiskirchen, S., & Weiskirchen, R. (2016). Resveratrol: How much wine do you have to drink to stay healthy? Advances in nutrition, 7(4), 706-718.

Willcox, J. K., Ash, S. L., & Catignani, G. L. (2004). Antioxidants and prevention of chronic disease. Critical reviews in food science and nutrition, 44(4), 275-295.

Wirasorn, K., Klarod, K., Hongsprabhas, P., & Boonsiri, P. (2014). Oxidative stress, antioxidant and cancer. Srinagarind Medical Journal, 29(2), 207-219. (in Thai)

Wongwanich, S., & Wiratchai, N. (2003). Thesis consultation guidelines. Bangkok: Textbooks and Academic Papers Center, Faculty of Education, Chulalongkorn University. (in Thai)

Yamasaki, K., Hashimoto, A., Kokusenya, Y., Miyamoto, T., & Sato, T. (1994). Electrochemical method for estimating the antioxidative effect of methanol extracts of crude drugs. Chemical and pharmaceutical bulletin, 42, 1663-1665.