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Oxidative stress is a status occurred by an imbalance between production and accumulation of oxygen reactive species (ROS) in cells and tissues that lead to oxidative damage of DNA, protein, fat molecules. It increases an inflammation and chronic diseases such as non-communicable diseases (NCDs) that mainly caused by unhealthy lifestyle and environment. Recently, dietary antioxidant supplementation is one of the most comfort way to decrease free radical content and support antioxidant activity. This study aimed to evaluate the effects of antioxidants activity on supplementary diet intake. The quasi-experimental study was conducted in 20 participants. Blood samples were obtained 2 times, before and after continuously daily dietary supplement intake for 14 days. Free radicals and antioxidant content were analyzed. The demographic data showed a total of healthy participants were enrolled with mean age of 34.90±9.58 years, body weight of 57.93±11.15 kilograms and 80% were female. The results showed a significant reduction of mean difference in free radical level of P=0.04 (95% CI, 1.23-49.37) and significant increase mean difference in antioxidant content of P<0.01 (95% CI, 0.03-0.15) under the effect of dietary supplement. This dietary supplement significantly reduced free radical and increased antioxidant content that may reduce oxidative stress in healthy working-age participants with continuous daily intake. However, lifestyle modification and randomized controlled trial should be recommended for additional evidence.
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