Types and Distribution of Calcium Oxalate Crystals in Leaves of Some Medicinal Plants in Ubon Ratchathani Province
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Abstract
Calcium oxalate (CaOx) crystals are ergastic bodies observed in over 215 plant families. Information of types and characteristics of CaOx crystals is useful for taxonomic identification of plant taxa and serves as an essential diagnostic tool for identification and detection of adulterants in crude drugs. This research was to study types and distribution of CaOx crystals in leaf samples of 18 medicinal plant species in Ubon Ratchathani province, classified into 18 genera and 15 families. Leaf blades and petioles of all samples were cross sectioned and stained with safranin. Types and distribution of CaOx crystals in the plants were examined by a bright field microscope. The distribution of CaOx crystals in leaf blades was examined in epidermis, mesophyll and vascular tissue while that in petioles was examined in epidermis, cortex and vascular tissue. The results revealed that most of the studied plants which were 13 species had druse crystals including Akschindlium godefroyanum (Kuntze) H.Ohashi, Buchanania siamensis Miq., Casearia flexuosa Craib, Catunaregam tomentosa (Blume ex DC.) Tirveng., Cyrtophyllum fragrans (Roxb.) DC., Getonia floribunda Roxb., Leea indica (Burm.f.) Merr., Memecylon edule Roxb., Microcos tomentosa Sm., Ochna integerrima (Lour.) Merr., Polyalthia evecta (Pierre) Finet & Gagnep., Salacia chinensis L. and Sphaerocoryne lefevrei (Baill.) D.M.Johnson & N.A.Murray. It was also found that 3 plant species had prismatic crystals and druse crystals including Artabotrys harmandii Finet & Gagnep., Suregada multiflora (A.Juss.) Baill. and Xylopia vielana Pierre. Dianella ensifolia (L.) Redouté and Tetracera scandens (L.) Merr. were the plant species having only prismatic crystals and raphide crystals, respectively. The distribution of CaOx crystals found in different plants varied depending on plant species and parts of plant leaves used in the study.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
References
Franceschi, V.R. and Nakata, P.A. 2005. Calcium oxalate in plants: formation and function. Annual Review of Plant Biology. 56: 41-71.
Franceschi, V.R. and Horner, H.T. Jr. 1980. Calcium oxalate crystals in plants. The Botanical Review. 46: 361-427.
Molano-Flores, B. 2001. Herbivory and calcium concentrations affect calcium oxalate crystal formation in leaves of Sida (Malvaceae). Annals of Botany. 88: 387-391.
Ilarslan, H., Palmer, R.G. and Horner, H.T. Jr. 2001. Calcium oxalate crystals in developing seeds of soybean. Annals of Botany. 88(2): 243-257.
Tilton, V.R. and Horner, H.T. Jr. 1980. Calcium oxalate raphide crystals and crystalliferous idioblasts in the carpels of Ornithogalum caudatum. Annals of Botany. 46: 533-539.
Cutter, E.G. 1978. Plant Anatomy: Cells and Tissues Pt. 1: Experiment and Interpretation (Contemporary Biology). 2nd edition. London: Hodder & Stoughton.
Esau, K. 1977. Anatomy of Seed Plants. 2nd edition. Hoboken: John Wiley & Sons, Inc.
Fahn, A. 1982. Plant Anatomy. 3rd edition. Oxford: Pergamon Press.
Genua, T.M. and Hillson, C.J. 1985. The occurrence, type and location of calcium oxalate crystals in the leaves of fourteen species of Araceae. Annals of Botany. 56: 351-361.
Ilarsan, H. and et al. 1997. Quantitative determination of calcium oxalate and oxalate in developing seeds of soybean (Leguminosae). American Journal of Botany. 84(8): 1042-1046.
Prychid, C.J. and Rudall, P.J. 1999. Calcium oxalate crystals in monocotyledons: a review of their structure and systematics. Annals of Botany. 84: 725–739.
Anitha, R. and Sandhiya, T. 2014. Occurrence of calcium oxalate crystals in the leaves of medicinal plants. International Journal of Pharmacognosy. 1(6): 389-393.
Odufuwa, T.K. and et al. 2014. Juicing alters oxalates contents in commonly consumed leafy vegetables in Southwest Nigeria. International Journal of Nutrition and Food Sciences. 3(3): 183-186.
Kartal, C. 2016. Calcium oxalate crystals in some species of the tribe Cardueae (Asteraceae). Botanical Sciences. 94(1): 107-119.
Munuswamy, E., Krishnan, V. and Amerjothy, S. 2016. Occurrence, type and location of calcium oxalate crystals in selected medicinal plants. Journal of Applied and Advanced Research. 1(4): 21-24.
El-Zaidy, M. and Alsahli, A.A. 2021. Occurrence of calcium oxalate crystals in some wild plants used in traditional medicine in Saudi Arabia. Journal of Medicinal Plants Research. 15(2): 96-107.
Capacio, A.F.Z. and Belonias, B.S. 2018. Occurrence and variation of calcium oxalate crystals in selected medicinal plant species. Annals of Tropical Research. 40(2): 45-60.
Huang, J., Huang, C. and Liebman, M. 2015. Oxalate contents of commonly used Chinese medicinal herbs. Journal of Traditional Chinese Medicine. 35(5): 594-599.
Kesonbua, K. and Kodkaew, Y. 2022. Leaf epidermal anatomy for the identification of some medicinal plant in Ubon Ratchathani University. Journal of Science and Technology, Ubon Ratchathani University. 24(1): 17-33. (in Thai)
Khoomgratok, S. and et al. 2006. Examination of calcium oxalate from indigenous vegetable. Agricultural Science Journal. 37(6) (Suppl): 189-192. (in Thai)
Piwpuan, N. 2014. Calcium oxalate crystals and oxalate content in some vegetables from Nong Khai province. KKU Science Journal. 42(4): 820-829. (in Thai)
Pennisi, S.V. and et al. 2002. Intercellular calcium oxalate crystal structure in Dracanena sanderiana. New Phytologist. 150(1): 111-120.
Meric, C. 2009. Calcium oxalate crystals in some species of the tribe Inuleae (Asteraceae). Acta Biologica Cracoviensia Series Botanica. 51(1): 105-110.
Wu, C.C. and Kuo-Huang, L.L. 1997. Calcium crystals in the leaves of some species of Moraceae. Botanical Bulletin of Academia Sinica. 38: 97-104.
Edeoga, H.O. and Ugbo, H.N. 1997. Histochemical localization of calcium oxalate crystals in leaf epidermis of some Commelina L. (Commelinaceae) and its Bearing on Taxonomy. Acta Phytotaxonomica et Geobotanica. 48(1): 23-30.
Franceschi, V. 2001. Calcium oxalate in plants. Trends in Plant Science. 6(7): 331.
Borrelli, N, Benvenuto, M.L. and Osterrieth, M. 2016. Calcium oxalate crystal production and density at different phenological stages of soybean plants (Glycine max L.) from the southeast of the Pampean Plain, Argentina. Plant Biology. 18(6): 1016-1024.
Klomklaeng, T.S. and Cha-um, S. 2019. Physiological, organic and inorganic biochemical changes in the leaves of Elephant Ear (Colocasia esculenta Schott var. aquatillis). The Horticulture Journal. 88(4): 499-506.
