Inhibition of Pythium aphanidermatum Causing Rhizome Rot in Ginger by Antagonistic Bacteria
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Abstract
Rhizome rot caused by pathogenic fungi adversely affects ginger yield. The use of biocontrol microorganisms to prevent and control this disease is an interesting and environment-friendly method. This research aimed to isolate pathogenic fungi causing rhizome rot in ginger and to screen bacteria capable of inhibiting the most virulent pathogenic fungus from the isolated fungi. In this study, 5 isolates of rhizome rot causing fungi, ZP1, ZP2, ZP3, ZP4 and ZP5, were isolated from the diseased parts of ginger. From the pathogenicity test in ginger, it was found that the most virulent pathogenic fungus was the isolate ZP4. By using morphological and molecular methods, the fungus isolate ZP4 was identified as Pythium aphanidermatum. From the screening of antagonistic bacteria from soil surrounding ginger plants, it was found that out of 73 selected bacterial isolates, only 11 isolates showed antifungal activity against P. aphanidermatum ZP4 by the dual culture technique among which 5 isolates produced bacteriocin to inhibit P. aphanidermatum ZP4. The antagonistic bacteria exhibited the highest activity against the fungal pathogen P. aphanidermatum ZP4 were the isolates CP-8f and CP-13e which were identified by 16S rDNA sequence analysis as Bacillus velezensis and Bacillus subtilis, respectively. Furthermore, both of them were found to produce proteinase, α-amylase, cellulase and pectinase. These findings indicated that Bacillus velezensis CP-8f and Bacillus subtilis CP-13e had potential to be safe and environment-friendly biological control agents against rhizome rot causing fungi.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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