Arsenic Phytoremediation of Submerged Soil Using Canna glauca(L.)

Abstract

           This research aimed to investigate the effect of inorganic arsenic including As (III) and As (V) accumulation on Canna glauca (L.) growth. It was measured by percentage of dry weight reduction. The other was to study influential factors on the total arsenic accumulation of C.glauca and their organs, and their phytoremediation properties consisted of arsenic accumulation translocation factor and bioconcentration factor. The experimental design was 3 × 4 factorial in the CRD plan with 12 treatment combinations and 3 replications, and the 2 treatment factors were As (III) and As (V) treated in soil, and control group, as well as growing periods being 15, 30, 45 and 60 days. According to the result, the percentage of C.glauca   dry weight planted in As (III) treated soil significantly (P-value = 0.000) decreased more than As (V) being their mean 54.30% and 34.27%. The influential factors of total arsenic accumulation on C.glauca were significant (P-value = 0.000) arsenic speciation and growing periods, and then multiple comparison of means by Dunnett’s T3 found that As (III) > As (V) > Control and 60> 45> 30> 15 days. Both factors had significant influence each other (P-value = 0.000). The effect factors of total arsenic accumulation in various organs of C.glauca by 3 × 4 × 4 factorial experiment in the CRD plan comprised significantly arsenic speciation, growing period, and organs of C.glauca (P-value = 0.000). In addition the two impact factors had significant influences (P-value = 0.000) including arsenic speciation * growing period, arsenic speciation * organs of C.glauca, and growing period * organs of C.glauca, and then three factors had a significant influence (P-value = 0.000). The multiple comparison of these mean factors found that As (III) > As (V) > Control, 60 > 45 > 30 > 15 days, and rhizome > root > pseudostem > leaf. Arsenic accumulation translocation factor of C.glauca in As (III) treated soil was higher than As (V) on 15, 30 and 45 days, and bioconcentration of C.glauca in As (V) treated soil were ​​greater than 1 on 45 and 60 days; Therefore C.glauca was classified as hyperaccumulator plants.