Effects of perilla mint (Perilla frutescens) extracted meal supplementation in diets of laying hens (late period) on productive performance, eggs quality and enrichment of fatty acid in yolk

Abstract

The experiment was conducted to examine the utilization of perilla mint (Perilla frutescens) extracted meal in laying hen diets. Two hundred and forty (Hisex Brown^®), sixty weeks of age were raised under ambient temperature and assigned in a completely randomized design (CRD) with four dietary treatments and four replications per treatment (n=15). Each treatment contains different levels of perilla mint extracted meal as 0, 10, 30 and 50 g/kg All birds were fed with diets containing 18% of crude protein and 2,850 kcal/kg (ME) of laying hen diets to meet nutrient requirements of poultry according to NRC (1994). Diets were restricted (110 g/h/d) throughout the 56 day experimental period and drinking water was offered ad libitum to the birds. The results showed that nutrient digestibility and blood parameters were not significantly different (P>0.05) among levels of perilla mint extracted meal. However, experimental diet with three levels of perilla mint extracted meal supplementation significantly increased hen day production, egg weight, egg mass, feed conversion ratio per 1 kg of egg, and yolk weight (P<0.05). Addition of perilla mint extracted meal significantly increased the total cholesterol and HDL (P>0.05) when compared with the control but did not affect the LDL (P>0.05). In addition, supplementing three levels of perilla mint extracted meal in diets resulted in significant increases in total cholesterol, MUFA, PUFA, and LC- PUFA in egg yolk, mainly oleic acid (C18:1n9), linoleic acid (LA) (C18:2n6), linolenic acid (ALA) (C18:3n3) and docosahexaenoic acid (DHA) (C22:6n3) (P<0.01). Furthermore,     a result also showed that three levels of perilla mint extracted meal in the diets significantly increased omega 3, omega 6, and omega 9 fatty acids and omega 3 per omega 6 ratio in egg yolks (P<0.01). The results implied that perilla mint extracted meal could be used as suitable alternative feedstuffs in laying hen diets and showed that the use of diet supplemented with                   30 g/kg of perilla mint extracted meal is effective in enhancing the DHA and omega-3 fatty acid enrich in yolk.