Bacterial Identification and Molecular Detection of Virulence Genes in Bacteria Isolated from Infant Feces

Abstract

This study aimed to identify and detect pathogenic genes in bacterial strains which were isolated from feces of healthy infants. The techniques used to detect the genes in this research are PCR technique and DNA sequencing. Bacterial species identification was performed by 16s ribosomal DNA amplification, sequencing and analysis. It was found that all bacterial isolates were identifies as Enterococcus faecalis. At least one pathogenic gene of the genes (cylA, ace, agg, esp and gelE) were found in all bacterial isolates. This research demonstrated that E. faecalis, a normal flora in human’s intestine, had pathogenic genes which could be transferred to other bacterial species.