Isolation and Probiotic Properties of Lactic Acid Bacteria Isolated from Raw Banana (Musa sapientum L.) in Phitsanulok Province
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Abstract
The objectives of this study were to isolate lactic acid bacteria (LAB) from raw bananas (Musa sapientum L.) in Phitsanulok Province and to study the probiotic properties of isolated LABs. The species of LABs were identified using molecular biology techniques. Probiotic properties of isolated LABs such as morphological characteristics, hemolytic activity, 0.5% bile salt tolerance, pH tolerance at 2-9, antimicrobial activity, and antibiotics activity were examined. Results revealed 35 strains of LAB isolated from raw bananas (gram-positive bacteria with catalase-negative properties). However, there were only seven isolated LABs i.e. NR19, NR22, NR23, NR24, NR27, NR28, and NR38 showed a low hemolytic activity. The 7 isolated LABs could resist 0.5% bile salt with over 90% survival rate as well as resist pH 2-9 with ≥ a 40% survival rate. There were four isolated LABs (NR19, NR23, NR24, and NR27) revealing the antibacterial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Salmonella Typhimurium. Moreover, the seven isolated LABs were sensitive to chloramphenicol and ampicillin. After sequence analysis of 16S rDNA genes of the isolated LABs, it was found that the seven isolated LABs were similar to Enterococcus gallinarum (NR19) Lactococcus lactis ssp. (NR22, NR23, NR24, and NR38) and Weisssella paramesenteroides (NR27 and NR28). Therefore, isolated LABs from raw bananas exhibited promising probiotic properties and could be further developed to food supplements.
Article Details
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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