Factors Influencing Mycelial Growth, Yield, Quality and ß-glucan Content of Hed Taen Rad (Macrocybe crassa)
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Abstract
ß-glucan belongs to bioactive polysaccharides found in a variety of mushrooms including Hed Taen Rad (Macrocybe crassa). ß-glucan contents in mushrooms depend on a number of factors such as mushroom strains and cultivation substrate compositions. This research aimed to study factors influencing mycelial growth, yield, quality and ß-glucan content of 5 M. crassa strains (DOA, DOA-1, DOA-4, DOA-7 and DOA-10). Factors influencing mycelial growth of M. crassa strains to be tested were types of media (Corn meal agar, Coconut water agar, Glucose peptone agar, V8 juice agar, Malt extract agar and Potato dextrose agar), pH of media (4, 5, 6, 7, 8, 9 and 10) and temperatures for mycelial growth (15, 20, 25, 30, 35 and 40oC). The results revealed that the optimal conditions for mycelial growth of all M. crassa strains used in this study were cultivation on Malt extract agar at pH in the range of 5-10 and at temperatures in the range of 20-30oC. Factors influencing yield, quality and ß-glucan content of M. crassa strains to be tested were 7 cultivation substrate formulas for mushroom cultivation in mushroom house condition. Mushrooms cultivated with different cultivation substrates were analyzed for their yields, quality of fruiting bodies, nutritional values and ß-glucan contents. The results demonstrated that cultivation of M. crassa strain DOA-10 with cultivation substrate formula 7 (old sawdust mixed with corn seed powder) resulted in the maximum yield of mushroom and the highest quality of fruiting body with the highest cap diameter and stalk length. On the other hand, cultivation of M. crassa strain DOA-1 with cultivation substrate formula 2 (standard formula for commercial mushroom cultivation) resulted in the maximum contents of ß–glucan and some major minerals (phosphorus, potassium and calcium). The information obtained from this study is useful for further development of the commercial production of M. crassa in the future.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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