Bactericidal Activity and Antagonistic Mechanism of Kojic Acid against Pseudomonas aeruginosa
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Abstract
Kojic acid is commonly used in cosmetic products to lighten the skin. Bacterial contamination is one of the serious problems in cosmetics because they contain ingredients that support the growth of microorganisms. Pseudomonas aeruginosa is not only the most frequently found in contaminated cosmetic products but also the major cause of skin infections. The aim of this study was to assess the effectiveness and the mechanism of kojic acid in inhibiting growth of P. aeruginosa. By swab paper disc method, it was found that kojic acid clearly inhibited P. aeruginosa. The broth dilution method revealed that the minimum inhibitory concentration (MIC) of kojic acid against P. aeruginosa was 1%. Kojic acid at the MIC level exhibited bactericidal effect on P. aeruginosa causing cell deformation examined by scanning electron microscopy and leakage of nucleic acids and proteins from P. aeruginosa cells evaluated by spectrophotometry at the wavelengths of 260 and 280 nm, respectively. The results may be useful as a guideline for determining the proper amount of kojic acid to be used in cosmetics for inhibition of bacteria.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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