Isolation of Xylanase-Producing Alkaliphilic Fungi and Development of Fungal Culture Media from Some Agricultural Crops
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Abstract
The objective of the present study was to isolate xylanase-producing alkaliphilic fungi from soil containing decayed agricultural waste and to develop fungal culture media from some agricultural crops for culturing the isolated fungi. In this study, a total of 23 alkaliphilic fungal isolates were isolated from soil containing decayed agricultural waste. The alkaliphilic fungal isolates were tested for their xylanase production ability using Gram’s iodine assay on xylan agar, pH 9 which was expressed as the enzymatic index calculated from the ratio of clear zone diameter to colony diameter. It was found that the fungal isolates GMCF 16 and GMSS 14 exhibited the highest xylanase activity with the enzymatic indexes of 2.07±0.25 and 1.94±0.08, respectively. Based on morphological characteristics, the two fungal isolates were identified belonging to the genus Aspergillus. The fungal isolates were subsequently cultivated on culture media prepared from 4 agricultural crops including cassava, purple sweet potato, corn and red kidney bean while in the control, they were cultivated on commercial potato dextrose agar (PDA). The initial pH of all fungal culture media was adjusted to 9. It was found that Aspergillus sp. GMSS 14 showed the highest growth on purple sweet potato agar, followed by commercial PDA, and the least growth on cassava agar, corn agar and red kidney bean agar whereas Aspergillus sp. GMCF 16 was able to grow well on all culture media. This research demonstrated the feasibility of developing new fungal culture media from agricultural crops to replace the relatively expensive commercial PDA commonly used in laboratories.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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