Evaluation of Virulence of Local Isolates of Entomopathogenic Fungi against Common Cutworm (Spodoptera litura Fabricius) Under Laboratory Condition and Spore Production Using Cereal Grains
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Abstract
This research aimed to evaluate the virulence of local isolates of entomopathogenic fungi against a common cutworm (Spodoptera litura Fabricius) under laboratory condition and to evaluate spore production using cereal grains. From the isolation of entomopathogenic fungi from soil samples collected from different areas in Nakhon Ratchasima province, a total of 11 isolates of Metarhizium spp. (MetNM_M4/3, MetNM_M7/3, MetNM_M8/6, MetNM_M10/2, MetNM_M11/1, MetNM_JKR14/2, MetNM_JKR16/3, MetNM_JKR17/2, MetNM_JKR18/4, MetNM_JKR19/4 and MetNM_NBM10/3) and 1 isolate of Beauveria bassiana (BbNM_CC1/1) were isolated. When all of the isolated entomopathogenic fungi were evaluated for their mycelium growth on potato dextrose agar (PDA) for 21 days, the isolate MetNM_M7/3 had the largest average colony diameter (78.00 mm) while the isolate B. bassiana isolate BbNM_CC1/1 had the smallest average colony diameter (45.50 mm). All of the isolated entomopathogenic fungi were tested for their infectivity against a common cutworm under laboratory condition by spraying fungal spores at a concentration of 1×108 spores/mL on the 3rd instar common cutworm. It was found that the isolate BbNM_CC1/1 caused the highest cumulative mortality and cumulative infection of the common cutworm (96.67%). When the isolate BbNM_CC1/1 was tested for its spore production on different cereal grains (sorghum, unmilled rice, maize, broken maize, and broken rice) by cultivating the fungus on the cereal grains for 7, 14 and 21 days, the fungus produced the highest number of spores on broken rice (4.10 x 108 spores/g) when cultivated for 21 days. This study demonstrated that the isolate B. bassiana BbNM_CC1/1 was the most virulent against a common cutworm under laboratory condition. Therefore, it would be interesting for further evaluation in greenhouse and field conditions.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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