Endophytic Fungi Producing Indole Acetic Acid and Promoting Seed Germination of Habenaria rhodocheila Hance
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Abstract
This research aimed to isolate endophytic fungi producing indole acetic acid (IAA) and promoting seed germination of Habenaria rhodocheila Hance. A total of 26 isolates of endophytic fungi were isolated from H. rhodocheila roots. By using Salkowski’s method to examine IAA production of the isolated endophytic fungi, only 6 isolates were found to be able to produce IAA including isolates HRNP-F01, HRNP-F06, HRNP-F14, HRNP-F17, HRNP-F21 and HRNP-F22. From co-culture of H. rhodocheila seeds with 6 isolates of IAA producing endophytic fungi, it was found that the isolates HRNP-F06 and HRNP-F14 exhibited the highest percentages of seed germination of 97.27% and 98.02%, respectively which were significantly different (p<0.05) from that of the control. The isolate HRNP-F06 was able to stimulate seeds to germinate and develop into stage 2 of seed germination (the stage at which embryo enlargement continues), while the isolate HRNP-F14 was able to stimulate seeds to germinate and develop into stage 3 of seed germination (the stage at which embryo develops into protocorm). Nucleotide sequence analysis of the internal transcribed spacer (ITS) region of ribosomal DNA identified the isolates HRNP-F06 and HRNP-F14 as Fusarium oxysporum and Annulohypoxylon sp., respectively. This study indicated that F. oxysporum HRNP-F06 and Annulohypoxylon sp. HRNP-F14 had potential to be used to promote germination of seeds.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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