การวิเคราะห์โพลีไซคลิกอะโรมาติกไฮโดรคาร์บอนในหมูปิ้งโดยใช้เทคนิคการสกัดด้วยคลื่นไมโครเวฟและการสกัดระดับจุลภาคร่วมกับโครมาโทกราฟีของเหลวสมรรถนะสูง

Abstract

                This work studied the determination of three polycyclic aromatic hydrocarbons (PAHs) namely fluorene, phenanthrene and anthracene in grilled pork using microwave-assisted extraction and microextraction combined with high performance liquid chromatography (HPLC). The parameters affecting all methods were investigated and optimized such as mobile phase compositions for HPLC method, type and volume of solvents for extraction methods. Under the optimum condition, the simultaneous analysis of three PAHs was achieved within 24 min. Limit of detections were 10-15 ppb. The linearity of the method was in the range of 0.02-10 ppm. The relative standard deviations (RSDs) were 2.72-3.51% for intra-day precision and 3.23-4.09% for inter-day precision. The recoveries of three PAHs ranged from 75.38 to 93.60%. The proposed method provides good precision and accuracy for the analysis of PAHs in grilled pork samples.