Karyotypic Study of Male Predaceos Spear-headed Katydid (Palaeoagraecia brunnea) and Male Bluish-green Rice Grasshopper (Hieroglyphus banian) in Northern Thailand
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Abstract
This study analyzed the karyotype and idiogram of male predaceos spear-headed katydid, Palaeoagraecia brunnea (Ingrisch, 1998), and male bluish-green rice grasshopper, Hieroglyphus banian (Fabricius, 1798), in Northern Thailand. Grasshoppers were collected from Lamphun and Chiang Mai Provinces. The mitotic chromosomes were directly prepared from gut tissue and male testis tissue by colchicine-hypotonic-fixation techniques. Then stained by conventional staining using 20% (v/v) Giemsa working solution. Chromosomes were observed under compound light microscope (1,000 times). The results showed that the diploid chromosome number of the male Palaeoagraecia brunnea was 2n = 13 including 12 autosomes and 1 X chromosome. The karyotype formula of the male Palaeoagraecia brunnea was deduced as: 2n (13) = Lm12 + X (Lm) chromosome. The diploid chromosome number of the male Hieroglyphus banian was 2n = 23 including 22 autosomes and 1 X chromosome. The karyotype formula of the male Hieroglyphus banian was deduced as: 2n (23) = Lt8 + Mt6 + St8 + X (Lt) chromosome.
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References
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21. Fox DP, Carter KC, Hewitt GM. Giemsa banding and chiasma distribution in the desert locust. Genetics 1985;31:272-276.
22. John B, Naylor B. Anomalous chromosome behavior in the germ line of Schistocerca gregaria. Heredit 1961;16:187-198.
23. Hemp C, Heller K, Warchalowska-Sliwa E, Grzywacz B. A molecular phylogeny of east African amytta (Orthoptera: Tettigoniidae, Meconematinae) with data on their cytogenetics. Systematic Entomology 2018; 43(2):239-249.
2. พิสุทธิ์ เอกอำนวย. โลกของตั๊กแตน จิ้งหรีด 2 ตั๊กแตนต่างๆ Grasshoppers. พิมพ์ครั้งที่ 1. กรุงเทพฯ: พลัสเพรส; 2556. หน้า 10-34.
3. Mesa A, Fontanetti CS. Karyotypes of nine Brazilian species of acridids (Orthoptera, Acridoidea). Rev Bras Genet 1983;6(2):295-305.
4. Na L, Bing-Zhong R. Karyotypes of Tettigoniidae (Orthoptera: Tettigonioidea) in Northeast China. Zootaxa 2007;1475:61-68.
5. Ferreira A, Mesa A. Cytogenetics studies in thirteen brazilian species of Phaneropterinae (Orthoptera: Tettigonioidea: Tettigoniidae): main evolutive trends based on their karyological traits. Neotropical entomology 2007;36(4):503-509.
6. Warchalowska-Sliwa E, Grzywacz B, Maryanska-Nadachowska A, Karamysheva TV, Rubtsov NB, Chobanov DP. Chromosomal differentiation among bisexual European species of saga (Orthoptera: Tettigoniidae: Saginae) detected by both classical and molecular methods. European Journal of Entomology 2009; 106(1):1-9.
7. Grzywacz B, Hemp C, Heller KG, Hemp A, Chobanov DP, Warchałowska-Sliwa E. Cytogenetics and molecular differentiation in the African armoured ground bushcrickets (Orthoptera: Tettigoniidae: Hetrodinae). Zoologischer anzeiger 2015;259(1):22-30.
8. Hemp C, Heller KC, Warchałowska-Śliwa E, Grzywacz B, Hemp A. Ecology, acoustics and chromosomes of the East African genus Afroanthracites Hemp & Ingrisch (Orthoptera, Tettigoniidae, Conocephalinae, Agraeciini) with the description of new species. Organisms Diversity & Evolution 2015;15:351-368.
9. Warchalowska-Sliwa E, Grzywacz B, Maryanska-Nadachowska A, Karamysheva TV, Chobanov DP, Heller KG. Cytogenetic variability among bradyporinae species (Orthoptera: Tettigoniidae). European Journal of Entomology 2013;110(1):1-12.
10. Barranco P, Cabrero J, Camacho JPM, Pascual F. Chromosomal basis for a bilateral gynandromorph in pycnogaster inermis (Rambur,1838) (Orthoptera, Tettigoniidae). Contribution to Zoology 1995;65(2):123-127.
11. Ferreira A, Mesa A. Cytogenetics studies in brazilian species of Pseudophyllinae (Orthoptera: Tettigoniidae): 2n(♂)=35 and FN=35 the probable basic and ancestral karyotype of the family Tettigoniidae. Neotropical entomology 2010;39(4):590-594.
12. Grzywacz B, Tatsuta H, Shikat K, Elżbieta W. A comparative chromosome mapping study in Japanese Podismini grasshoppers (Orthoptera: Acrididae: Melanoplinae). Cytogenetic and Genome Research 2018;154(1):37-44.
13. Bugrov A, Warchalowska-Śliwa E, Maryańska -Nadachowska A. Karyotype evolution and chromosome C-banding patterns in some podismini grasshoppers (Orthoptera, Acrididae). Caryologia 1994;47(2):183-191.
14. บังอร กองอิ้ม. ไมโตติกคาริโอไทป์ของตั๊กแตนหนวดสั้นบางชนิดในจังหวัดขอนแก่นและสกลนคร. วิทยานิพนธ์ปริญญาวิทยาศาสตรมหาบัณฑิต สาขาวิชาชีววิทยา, บัณฑิตวิทยาลัย มหาวิทยาลัยขอนแก่น. ขอนแก่น; 2544. หน้า 108-109.
15. Ashok KS. Cytology and cytotaxonomy of Acrididae: a summary. Rec zool Surv India 2006;106(3):47-78.
16. Koli YJ, Gaikwad SM, Bharmal DL, Bhawane GP. Karyotypic studies of six species of grasshopper (Orthoptera: Acrididae) from Kolhapur District, Maharashtra, India. Cytologia 2013;78(3):255-260.
17. Phimphan S, Sangpakdee W, Sangpakdee K, Tanomtong A. Chromosomal analysis and meiosis studies of Oxya chinensis (Orthoptera: Acrididae) from Thailand. The Nucleus 2017; 60(1):9-15.
18. Sandhu SK, Chadha P. Karyological studies of four species of grasshoppers from Gurdaspur District of Punjab, India. The Nucleus 2012;55(3):167-170.
19. อลงกลด แทนออมทอง กฤษณ์ ปิ่นทอง และอิสสระ ปะทะวัง. พันธุศาสตร์ระดับเซลล์. พิมพ์ครั้งที่ 1. กรุงเทพฯ: สำนักพิมพ์จุฬาลงกรณ์มหาวิทยาลัย; 2562. หน้า 429-505.
20. Turpin R, Lejeune J. Les chromosomes humains (caryotype normal et variations pathologiques). Paris: Gauthier Villars 1965;(6): 965-966.
21. Fox DP, Carter KC, Hewitt GM. Giemsa banding and chiasma distribution in the desert locust. Genetics 1985;31:272-276.
22. John B, Naylor B. Anomalous chromosome behavior in the germ line of Schistocerca gregaria. Heredit 1961;16:187-198.
23. Hemp C, Heller K, Warchalowska-Sliwa E, Grzywacz B. A molecular phylogeny of east African amytta (Orthoptera: Tettigoniidae, Meconematinae) with data on their cytogenetics. Systematic Entomology 2018; 43(2):239-249.