The Production of Bacillus sp. Cell Biomass at High Concentration by Special Batch Cultivation Technique Instead of Fed-Batch Mode

Abstract

The production of Bacillus sp. cell biomass at high concentration with the defined medium using dextrin (glucose oligomer) from cassava starch hydrolysis at high concentrations as the carbon source together with the batch cultivation technique, which is simple and inexpensive. The study in flask using the Batch Production Medium (BPM) with dextrin as the carbon source showed the efficient biomass yield of 12.93 g/l. Furthermore, in 5-liter fermenter cultivation, dextrin could be used at a very high concentration up to 100 g/l without growth inhibition (it cannot be done, if using sugar as the carbon source) and enhanced cell biomass concentration to 31.98 g/l. The further scaled-up studies of the cultivations in 30 and 300-liter fermenters were carefully done in order to test the feasibility for the production at the industrial scale. They were clearly showed the excellent results because of good cell growths and giving biomass yields at the very high concentrations of 43.45 and 47.03 g/l at the very high productivities (production rates) of 3.11 and 3.92 g/l/h, respectively. The results of this study importantly demonstrate the highly effective production which is able to develop to the industrial scales and substitute the usual fed-batch cultivation technique which is the complicated method and high cost.