องค์ประกอบทางเคมี ความเป็นพิษต่อเซลล์ ฤทธิ์ยับยั้งการผลิตไนตริกออกไซด์ และการทำงานของเอนไซม์แอลฟา-กลูโคซิเดส และฤทธิ์ต้านอนุมูลอิสระของสารสกัดใบหนานเฉาเหว่ย (Vernonia amygdalina Del.)
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มี.ค. 3, 2022
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สารสกัดใบหนานเฉาเหว่ย เอนไซม์แอลฟา-กูลโคซิเดส ต้านอนุมูลอิสระ ต้านการอักเสบ
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บทคัดย่อ
การศึกษาองค์ประกอบทางเคมี ความเป็นพิษต่อเซลล์ ฤทธิ์ยับยั้งการสร้างไนตริค ออกไซด์และการทำงานของเอนไซม์แอลฟา-กลูโคซิเดส และฤทธิ์ต้านอนุมูลอิสระ ของสารสกัดใบหนานเฉาเหว่ย พบสารที่เป็นองค์ประกอบในสารสกัด 10 ชนิด ได้แก่ Benzoic acid, Neophytadiene, Xylocaine, Hexadecanoic acid, Hexadecanoic acid ethyl ether, Phytol, (z,z)-9,12-octadecadienoic acid, (z,z,z)-9,12,15-octadecatrien-1-ol, (z,z,z)-9,12,15-octadecatrienoic acid, ethyl ester และ octadecanoic acid สารสกัดค่อนข้างเป็นพิษต่อเซลล์ โดยมีค่า IC50 ที่ 110.02 ± 0.86 µg/ml และที่ความเข้มข้น 100 µg/ml สารสกัด ยับยั้งการสร้างไนตริกออกไซด์ได้ 17.39+2.41% แต่น้อยกว่า Diclofenac ที่ยับยั้งได้ถึง 27.07+1.81% สารสกัด ยังยับยั้งการทำงานของเอนไซม์แอลฟา-กลูโคซิเดสได้ โดยมีค่า IC50 เท่ากับ 0.275 mg/ml ซึ่งยับยั้งได้น้อยกว่า Acarbose ที่มีค่า IC50 เท่ากับ 0.157 mg/ml นอกจากนี้ สารสกัดยังกำจัดอนุมูล DPPH ได้ และการกำจัดผันแปรตามความเข้มข้นของสารสกัด โดยความเข้มข้นสูงสุดที่ใช้ คือ 1.6 mg/ml กำจัดได้ 35.53+0.03% ขณะที่ สาร BHT กำจัดได้ 70.37+0.00% ชี้ให้เห็นว่า มีความเป็นไปได้ ที่จะนำสารสกัดใบหนานเฉาเหว่ย ไปพัฒนาและประยุกต์ใช้รักษาโรคที่สัมพันธ์กับอนุมูลอิสระ เช่น โรคเบาหวาน และการอักเสบได้
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References
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2. Mlatovi Dégbé, Françoise Debierre-Grockiego, Amivi Tété-Bénissan, Héloïse Débare, Kodjo Aklikokou, Isabelle Dimier-Poisson, and Messanvi Gbeassor. 2018. Extracts of Tectona grandis and Vernonia amygdalina have anti-Toxoplasma and pro-inflammatory properties in vitro. Laboratoire de Physiologie et de Pharmacologie des Substances Naturelles, Faculté des Sciences, Université de Lomé, B.P. 1515, Lomé 01, Togo. ISP, INRA, Université Tours, 37380 Nouzilly, France
3. Hutchioson and Dalzell J. M. (1963). Flora of West Africa (2nd edition) agent London pp 450 – 455.
4. Yeap SK, Yong HW, Beh BK, et al. 2010. Vernonia amygdalina, an ethnoveterinary and ethnomedical used green vegetable with multiple bioactivities. J Medic. Plants res., 4: 2787-2800
5. Yusmazura Zakaria, Nurhazirah Zainul Azlan, Nik Fakhuruddin Nik Hassan, Hussin Muhammad. In vivo antidiabetic efficacy of Malaysian Vernonia amygdalina aqueous extract. J Med Plants Stud 2018;6(4):72-77.
6. Reuben Okoduwa SI, Umar IA, James DB, Inuwa HM. Validation of the antidiabetic effects of Vernonia amygdalina Delile leaf fractions in fortified diet-fed streptozotocin-treated rat model of type-2 diabetes. J Diabetol 2017;8:74-85.
7. Item Justin Atangwho - Patrick Ekong Ebong - Eyong Ubana Eyong. Synergistic antidiabetic activity of Vernonia amygdalina and Azadirachta indica: Biochemical effects and possible mechanism. Journal of Ethnopharmacology, 141(3), 878-887 - April 2012
8. Abebe W. Traditional pharmaceutical practice in Gondar region, northwestern Ethiopia. J. Ethnopharmacol. 1984; 11:33-47.
9. Igile GO, Oleszek W, Jurzysta M, Burda S, Fanfunso M, Fasanmade AA. Flavonoids from Vernonia amygdalina and their antioxidant activities. J. Agric. Food Chem., 1994; 42:2445-2448.
10. J.a. Saliu, a.o. Ademiluyi, a.j. Akinyemi, g. Oboh. In vitro antidiabetes and antihypertension properties of phenolic extracts from bitter leaf (vernonia amygdalina DEL.) Journal of Food Biochemistry Volume36, Issue5 October 2012 Pages 569-576 First published: 22 December 2011
11. Tonukari, N.J., Avwioroko, O.J., Ezedom, T. and Anigboro, A.A. (2015) Effect of Preservation on Two Different Varieties of Vernonia amygdalina Del. (Bitter) Leaves. Food and Nutrition Sciences, 6, 623-632.
12. Divneet Kaur, Navpreet Kaur and Anuja Chopra. A comprehensive review on phytochemistry and pharmacological activities of Vernonia amygdalina. Journal of Pharmacognosy and Phytoc.hemistry 2019; 8(3): 2629-2636.
13. Ebenezer O. Farombi and Olatunde Owoeye. Antioxidative and Chemopreventive Properties of Vernonia amygdalina and Garcinia biflavonoid. Int. J. Environ. Res. Public Health 2011, 8, 2533-2555; doi:10.3390/ijerph8062533
14. Ijeh II, Ejike CE. Current perspectives on the medicinal potentials of Vernonia amygdalina Del. J Med Plants Res. 2011; 5(7):1051–61.
15. Owolabi MA, Jaja SI, Oyekanmi OO, Olatunji OJ. Evaluation of the antioxidant activity and lipid peroxidation of the leaves of Vernonia amygdalina. J Complement Integr Med. 2008; 5(1).
16. L. L. Hamman, D. S. Amaza, J. V. Zirahei, A. D. T. Goji, H. Mari and F. Amali (2016). Effect of aqueous extract of bitter leaf (Vernonia amygdalina) on phenyl-hydrazine induced kidney damage in albno rat. Int. J. Adv. Res. 4(11), 39-47 IJAR, 2016
17. Tona L, Cimanga RK, Mesia K, Musuamba CT, Bruyne T, Apers S, et al. In vitro antiplasmodial activity of extracts and fractions of seven medicinal plants used in the Democratic Republic of Congo. J. Ethnopharmacol. 2004; 93:27-32.
18. Udensi EA, Ijeh II, Ogbonna U. Effect of traditional processing on the phytochemical and nutrient composition of some local Nigerian leafy vegetables. J. Sci. Tech., 2002; 8:37-40.
19. Oluwaseun Ruth Alara, Nour Hamid Abdurahman, Chinonso Ishmael Ukaegbu & Nassereldeen Ahmed Kabbashi (2019) Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwaveassisted extraction techniques, Journal of Taibah University for Science, 13:1, 414-422,
20. Anoka A. Njan, Bulus Adzu, Amon G. Agaba, Dominic Byarugaba, Silvia Díaz-Llera, and David R. Bangsberg. The Analgesic and Antiplasmodial activities and toxicology of Vernonia amygdalina. Journal of Medicinal Food.Sep Vol. 11, No. 3, 2008.574-581
21. Immaculate Nabukenya. Acute and sub-acute toxicity of ethanolic and aqueous extracts of Tephrosia vogelii, Vernonia amygdalina and Senna occidentalis in rodents. 2nd International Conference and Exhibition on Traditional & Alternative Medicine August 25-26, 2014 DoubleTree by Hilton Beijing, China.
22. Ibrahim, G. & Abdulrahman, EM & Ibrahim, Hazem & Ibrahim, NDG & Magaji, Mohammed. (2011). Toxicity and analgesic effects of Vernonia amygdalina Del. (Asteraceae) leaf extract on mice. International Journal of Advanced Pharmaceutical and Biological Sciences. 1. 1-4. 10.1201/b11704-2)
23. Okwuzu, J. O., Odeiga, P., AdetoroOtubanjo, O., & Ezechi, O. C. (2017). Cytotoxicity testing of aqueous extract of bitter leaf (Vernonia amygdalina Del) and sniper 1000EC (2,3 dichlorovinyl dimethyl phosphate) using the Alium cepa test. African health sciences, 17(1), 147–153.
24. Olatunde OWOEYE, Sammer YOUSUF, Mohammad NADEEM AKHTAR, Kehkashan QAMAR, Ahsana DAR, Ebenezer Olatunde FAROMBI, Silas Kalu ONWUKA and Mohammad IQBAL CHOUDHARY. Another anticancer elemanolide from Vernonia amygdalina Del. J. Biol. Chem. Sci. 4(1): 226-234, February 2010 ISSN 1991-8631
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