การศึกษา Streptomyces sp. WPN31 ที่แยกได้จากดินบริเวณรากต้นเตยหอม (Pandanus amaryllifolius): การสร้างเอนไซม์อะไมเลส เซลลูเลส และไซลาเนส และการตรวจสอบคุณสมบัติการยับยั้งการสร้างไบโอฟิล์ม
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บทคัดย่อ
ไบโอฟิล์มเป็นสารที่ซับซ้อน ซึ่งหลั่งออกมาจากเชื้อจุลินทรีย์และสามารถเกาะติดกับพื้นผิวได้ ก่อให้เกิดการปนเปื้อนในเนื้อเยื่อของสิ่งมีชีวิต สิ่งแวดล้อม โรงงานอุตสาหกรรม และอุปกรณ์ทางการแพทย์ และยังเป็นปัจจัยที่สำคัญในการดื้อยาปฏิชีวนะของเชื้อจุลินทรีย์ การศึกษาครั้งนี้มีวัตถุประสงค์เพื่อตรวจสอบความสามารถของเชื้อ Streptomyces sp. WPN31 ที่แยกได้จากดินบริเวณรากต้นเตยหอม ในการสร้างเอนไซม์อะไมเลส เซลลูเลส และไซลาเนส และการยับยั้งการสร้างไบโอฟิล์มของแบคทีเรียก่อโรค โดยผลของยีน 16S rRNA พบว่า WPN31 มีความคล้ายคลึงกับ Streptomyces griseicoloratus (ร้อยละ 98.90) มากที่สุด ซึ่งเป็น streptomycetes สปีชีส์ใหม่ที่แยกได้จากดินในไร่ฝ้าย สาธารณรัฐประชาชนจีน อย่างไรก็ตาม พบว่า Streptomyces sp. WPN31 มีความสามารถในการผลิตเอนไซม์นอกเซลล์ ได้แก่ เอนไซม์อะไมเลส เซลลูเลส และไซลาเนสได้ที่อุณหภูมิ 37 และ 40 องศาเซลเซียส และจากการประเมินการยับยั้งการสร้างไบโอฟิล์มของ Staphylococcus aureus และ Pseudomonas aeruginosa โดยใช้สารส่วนเหนือตะกอนของ WPN31 พบว่า ส่วนเหนือตะกอนมีประสิทธิภาพในการป้องกันการสร้างไบโอฟิล์มของ S. aureus และ P. aeruginosa ดังนั้น การศึกษานี้จึงเป็นการรายงานเบื้องต้นเกี่ยวกับฤทธิ์ในการยับยั้งไบโอฟิล์มของ Streptomyces sp. WPN31 ซึ่งสามารถนำไปประยุกต์ใช้ในอุตสาหกรรมที่หลากหลายได้
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