Prevalence and diversity of Wolbachia in wild mosquito (Diptera: Culicidae) populations from the lower central Thailand
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Abstract
Mosquitoes are medically important insects, recognized as vectors for many serious diseases. Previous studies have
shownthat mosquitoesoftenharborendosymbioticbacteria, suchas Wolbachia, withintheir cells.Theseendosymbiotic
bacteriaplay vital roles in mosquitobiology, includingtheevolutionof various mosquitospecies. Consequently, humans have utilized Wolbachia to control mosquito populations, thereby reducing the spread of mosquito-borne diseases. However, data regarding the prevalence of Wolbachia symbiosis in mosquito populations in Thailand remains limited. This study aimed to investigate the prevalence and diversity of Wolbachia in mosquito populations inhabiting the lower central region of Thailand. A total of 144 mosquito samples were collected and identified as belonging to four genera and six species: Aedes sp., Ae. albopictus, Anopheles sp., Culex sp., Cx. quinquefasciatus, and Udaya argyrurus. Three mosquito species tested positive for Wolbachia using PCR assays: Ae. albopictus, Culex sp., and Cx. quinquefasciatus. Phylogenetic analysis of the Wolbachia 16S rRNA gene revealed that all strains belonged to supergroup B, with diversity varying among mosquito species. The findings of this research contribute to a better understanding of mosquito evolution influenced by Wolbachia. Furthermore, this knowledge provides a basis for the effective control of these insect vectors.
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References
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