Screening of antimicrobial-producing actinobacteria from peat soils against Candida albicans

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Published: Aug 26, 2026
Keywords:
Actinobacteria Candida albicans anticandidal activity Streptomyces

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Jetsda Tongtoht
Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus
Chonticha Benpard
Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus
Jintanart Wongchawalit
Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus
Ratchanee Mingma
Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus

Abstract

Yeast Candida albicans is the most frequent pathogen responsible for mucosal and systemic infections. Furthermore, it is the primary cause of approximately 70% of fungal infections globally. Microorganism-derived compounds are gaining attention as a promising alternative approach for controlling fungal diseases. The use of these compounds to combat C. albicans, which may develop drug resistance during therapy, is particularly promising. This study aimed to assess the anticandidal activity of actinobacteria obtained from peat soils against C. albicans CBS 562. Seventeen tested actinobacterial isolates exhibited anticandidal activity on starch casein agar and glucose yeast extract malt extract agar using the agar overlay method. The isolates 11A3 and 41N6, which showed the highest activity, were selected for evaluating the anticandidal efficacy of their crude extracts. The crude extracts of 11A3 and 41N6 demonstrated inhibitory effects on C. albicans CBS 562, with identical minimum inhibitory concentration (MIC) values of 3.125 mg/mL and minimum fungicidal concentration (MFC) values of 6.250 mg/mL and 3.125 mg/mL, respectively. Based on 16S rRNA gene sequence analysis, isolates 11A3 and 41N6 belong to the genus Streptomyces, showing the highest sequence similarity to Streptomyces marinus NBRC 12799T (99.54%) and Streptomyces malaysiense MUSC 136T (99.37%), respectively. This research indicates that actinobacteria have a strong potential to inhibit C. albicans, suggesting applications in biotechnology and pharmaceuticals.

Article Details

Issue
Vol. 45 No. 4 (2026): Journal of Science and Technology Mahasarakham University
Section
Original Articles

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