รูปแบบการแสดงออกของยีน Heat Shock Protein ในข้าวก่ำพะเยาระยะต้นกล้าภายใต้สภาวะแล้ง
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Abstract
Study of the drought stress effect on growth and expression of heat shock proteins in Khao Kum Phayao (Oryza sativa L.). Rice seedlings were germinated and grown for 7 days. Then 7-day old seedlings were continually grown in liquid Hoagland medium supplemented with 5 and 10 % (w/v) of PEG6000 to simulate drought stress and in Hoagland solution without PEG6000 (control) for a total of 21 days. The seedling growths were determined by the length of root and stem. The result showed that stem length in drought stress was shorter than control. While root length was longer compared to control. The pattern of gene expression of HSP90s (HSP50.2, HSP74.8 and HSP80.2), HSP70s (HSP23.7, HSP58.7 and HSP71.1) and small HSPs (HSP17.0, HSP24.1 and HSP26.7) were analyzed using semi-quantitative RT-PCR (sqRT-PCR) method. The HSPs expressions in seedling were exhibited in different expression patterns. The HSP58.7 expression was significantly induced in drought stress compared to the control. The HSP74.8 and HSP80.2 were constitutively expressed in drought condition and control. Moreover, HSP17.0 and HSP71.1 increasingly expressed following the period of seedling growth. The expression of both genes were independent of drought effect. Last group of HSPs as HSP23.7, HSP24.1, HSP26.7 and HSP50.2 showed that the effect of drought stress altered the pattern of gene expression by accelerating time of the maximum gene expression to be faster than control.
Keywords: drought stress; heat shock protein; Khao Kum Phayao
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