การประยุกต์อุปกรณ์ตรวจวัดสีแบบแอลอีดีสำหรับประเมินความสามารถในการต้านออกซิเดชันโดยรวมด้วยวิธีรีดิวซ์เฟอร์ริก
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Abstract
Abstract
This work presents an in-house colorimetric detector based on paired emitter detector diode (PEDD). The colorimetric detector composed of a pair of light emitting diodes (LED). The first LED was used as a light emitter and the second LED was used as a light detector. Then, the constructed colorimetric detector was coupled with a flow injection analysis (FIA) system in order to evaluate the total antioxidant capacity (TAC) by means of ferric reducing antioxidant power (FRAP) assay. When the antioxidant acts as reducing agent to facilitate the reduction of ferric ions, the color change of dark blue solution was monitored. Parameters affected the flow performance, which are color of LED, flow rate and injection volume, were also optimized. The FIA-PEDD system illustrated good performance with linearity range of 10-50 µM. Moreover, high throughput of 90 samples/h was achieved. The proposed method was applied in the analysis of the TAC values of commercial instant herbal beverages. According to the standard ascorbic acid, the TAC value was reported as ascorbic acid equivalent (AAE). It was found that AAE values obtained from our method were comparable with those obtained from the batch methods with a common spectrophotometric detector.
Keywords: paired emitter detector diode (PEDD); flow injection analysis (FIA); ferric reducing antioxidant power (FRAP); total antioxidant capacity (TAC)
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References
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