ผลของสีแคลลัสและระยะเวลาในการเพาะเลี้ยงต่อปริมาณสารทุติยภูมิของแคลลัสกระเจี๊ยบแดง
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Abstract
Color and culture period of callus play important roles on secondary metabolite contents of medicinal plants. The objectives of this study were to investigate the effects of different colors of roselle calli and callus culture periods of dark-red colored-callus on contents of total anthocyanin, total phenolic and total flavonoid including DPPH radical scavenging activities. The secondary metabolite contents of different colored-calli: green-white, red and dark red was determined. The results revealed that the dark-red colored-callus contained higher total anthocyanin content (0.38±0.08 mg cyd-3-glu/L), total phenolic content (28.29±3.16 mg GAE/g dry extract), flavonoids content (13.22±2.11 mg CE/g dry extract) and greater DPPH radical scavenging activity (EC50 = 78.82±6.61 µg/mL) than the red and green-white colored-calli. At different culture periods (7-28 days), it was found that the dark-red colored-callus cultured for 28 days contained the higher contents of total anthocyanin (0.45±0.08 mg cyd-3-glu/L), total flavonoid (11.34±2.60 mg CE/g dry extract) and the greatest DPPH radical scavenging activity (EC50 = 85.70±12.86 µg/mL) than those cultured for 7-21 days. However, there was no significant difference in total phenolic contents at all culture periods.
Keywords: callus; Hibiscus sabdariffa; anthocyanin; phenolic; flavonoid
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References
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