Inhibition of Listeria monocytogenes by Bacteriocin-like Substance Produced by Leuconostoc lactis BAN1.1
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Abstract
Leuconostoc lactis BAN1.1,a lactic acid bacterium isolated from banana peel, produces bacteriocin-like substance which could inhibit the important foodborne pathogen; Listeria monocytogenes. The bioactive compound was sensitive to proteolytic enzyme, confirming its proteinaceous nature. This research aim was to optimize the condition that was suitable for bacteriocin-like substance production. The results found that the growth medium consisted of 2% fructose, 2 % yeast extract, 0.2 % KH2PO4 and 0.2 % Tween 80, enhanced maximum antibacterial activity of bacteriocin-like substance, with the optimum temperature and pH as 25-30 oC and 6.5, respectively. In addition, it was found that bioactive compound was concentrated and showed strong inhibitory increasing almost two times in the dialysis step. This compound was stable over wide ranges of pH and high temperature. The effect of soaking with bacteriocin-like substance solution on survival of L. monocytogenes was also evaluated in fresh lettuce. The results showed that this substance decreased L. monocytogenes counts by 3.49±0.07 log CFU/g. These results revealed that the high potential of bacteriocin-like substance could be used as biopreservative in fresh fruit and vegetable products.
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