Development of Autologous Serum Eyes Drops Preparing Processes for Patients with Ocular Surface Disease

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Nichcha Pairatana
Wimolwan Tangpagasit Juwatanasumran
Pramote Sriwanitchrak
Kridsada Sirisabhabhorn


The formal processes for producing autologous blood serum eye drops (ASED) include a 2-hour incubation time and a 15-minute centrifugal time. Therefore, the main objectives of this Quasi-experimental study (manipulation and control only, without randomization) are (1) to reduce the processing time of the standard procedures of preparing the autologous serum eye drops, and (2) to compare the biochemical properties (pH and osmolality) of ASED from the developed processes with those of the standard one.  After establishing the developed processes with a 0.5-hour incubation time and a 5-minute centrifugal time and observing the experimental data, the ANOVA statistic method, Kruskal-Wallis Test, and Bland-Altman Plot, were used to analyze the data.

The experimental results show that the pH and osmolality values of ASED from the developed processes and those of the standard procedure are similar, and the insignificant differences are acceptable.  The pH and osmolality are 7.5-7.7 and 289-296 mmol/L, respectively, which are in the standard range.  Therefore, it can be concluded that the developed processes can be implemented, resulting in a reduction of waiting time from 4 hours to 1.5 hours and allowing patients to receive autologous serum eye drops faster than the previous procedures.

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Medical Sciences


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